Phytase enzyme which are produced commercially for utilization and improve nutrient digestibility added to poultry feeds. In the poultry feed industry ideal phytase enzyme is acidic pH unaffected and in the small intestine and stomach, where is cost-effective to produce, phosphorus absorption takes place and high temperatures resistant (65˚C - 80˚C), through feed pelleting are encountered, Lei X, Stahl C. (2001). In many others scientist and scholar’s research focused on the testing of efficacy and identification of the former for use of phytase enzyme in the animal feed industry ability to hydrolyze Phytic acid in the gastro intestinal tract [2c].

In poultry the site activity of microbial phytase enzyme at tract of poultry gastro intestinal sections. In proventriculus and crop followed by the jejunum and duodenum of poultry have greater activity of a microbial phytase (fungal phytase).

Two hundred day-old Ross chicks (Gallus gallus domesticus) were purchased from a commercial distributor hatchery of Hyderabad. After initial weight the chicks were first brooded together on deep litter system for one week. Chicken were arbitrarily separated in four was offered 0 g/kg group A (control), 0.05 g/kg (group B), 0.075 g/kg (group C) and 0.25 g/kg (group D) phytase enzyme provided in broiler feed. Each group were consisted of 50 birds these are presented (Table 1).

Feed was given twice a day, the feed which refused was collected daily to each group chickens ad libitum, Figure 1.

Before the research work, by electric weight balance chicken were weighed. In every week the birds were randomly selected from each group and weighed during experimental duration, Figure 2.

To calculate FCR, increasing weight and intake of feed, were noted by following formula;

FCR = Total feed consumed Total weight gain × 100

Analysis of variance showed that there were significant differences between the weight of birds amongst the four treatments (P < 0.01). The results of body weight of birds were noted in treated groups which were supplemented of phytase enzyme compared with un-supplemented group A. The maximum chicken body weight was noted in group C, as compare to group B. The average body weight was further decreased in group D and minimum in group A (control). The presence of phytase enzyme in group C diet recorded significantly (P < 0.05) the maximum body weight gain Figure 3(A). In instances where phytase enzyme is supplemented, the higher body weight gain may be attributable to an increase in P availability and, maybe feed intake, [13]; the higher CP (crude protein) content may be due to greater content of crude fiber (CF), which may impair nutrient absorption and digestion while the reduced weight gain of broilers fed the control diet group A may be ascribed to low crude protein content of the diet compared to other diets.

The intake of feed was analysis in different groups of chicks the feed intake was maximum in group A, as compare to treatments groups. The average intake of feed was further decreased in group C. Feed intake was minimum in group C. The results showed that in group a feed intake was higher than group C, B and D, Figure 3(B). In view of investigation the visible increase in feed intake might be attributed to increased largeness of the feed and metabolizable energy concentration of the diets. In instances where phytase enzyme is added, to rise in P availability and, may be feed intake.

The FCR of different groups were examined and the FCR was minimum in treatment group C and in group B was higher than treatments groups the non-significantly variance founded respectively, Figure 3(C). This was in agreement with the non-conventional feed stuff often decreases feed cost. This confirms that there is better economic gain by feeding phytase enzyme to broilers since it has the potential of reducing feeding cost of broilers. This supports the conclusion of several researchers that leaf meal supplementation in poultry rations has been proved as means of reducing cost and improving profit margin.

From four dietary treatments show (Figure 4), that there is Statistical analysis showed that group A and group D were significant difference in relative weight of liver compare to group B and C, while group B and C non-significant different from one another but with increase percentage of phytase enzyme, slightly weight of liver increased. That there were no visible differentiation throughout treatment except for spleen, lung, gizzard their weight was higher significantly in chicks provided phytase enzyme. Such morphological changes, supposedly caused by plant feed additives may provide further information on possible benefits to the digestive tract [14]; these results shows non-significant different in heart weight in different treated groups reported that heart weight slightly increased in different treated groups. The intestine weight of different groups analyzed and the intestine weight of groups D was higher than rest of groups. Such morphological changes in gastrointestinal tissues may provide further information on possible profits to the digestive tract [15]; the increase of villous height of different small intestine segment may be attributed to the role of the intestinal

epithelium as a natural barrier against pathogenic bacteria and toxic substances that are present in the intestinal lumen [16].

Level of blood profile of broiler was analysis, where the red blood cells, Hemoglobin, total protein, packed cell volume were counted higher in treated C group as related others treated group and control (A), respectively. The level of white blood were counted highest in D group and glucose was highest in (A) group compared to other treatments groups respectively.

All-pair-wise test shows that in two different groups (B and C) in which the means were non-significantly different from one another while significant different from control group A and group D, respectively. In Figure 5 (1), shows that white blood cells average were counted in group A (control) and treated groups (12.33, 12.48, 12.46 and 12.51 × 10³/µL), respectively. The comparable WBC of birds suggests that the animals were healthy because decrease in number of WBC below the normal range is an indication of allergic conditions, anaphylactic shock and certain parasitism or presence of foreign body in circulating system [17]; the general non significance of the WBC across treatments indicates that the experimental diets neither impaired nor enhanced the birds’ ability to wade off infection.

The result in Figure 5 (2), indicate that average red blood cells were count in different groups A, B, C, and group D (3.16, 3.29, 3.33 and 3.19 × 10⁶/µL), respectively.In group C the Maximum red blood cells was counted (3.33 × 10⁶/µL), as compared to group B (3.29 × 10⁶/µL) and group D (3.19 × 10⁶/µL). In group A

(control) the average was counted (3.16 × 10⁶/µL). Statistically there were no significant pairwise differences among the means of different groups, respectively. In group C the results shows the higher red blood cells counted than treated and control group A respectively. The values obtained for RBC of birds fed phytase enzyme diets were higher than the range of 3.07 to 7.50 × 10⁶/mm³ reported by [18]; but within 5 - 8 × 10⁶/mm³ reported by [19]; Red Blood Cells (RBC) are responsible for the transportation of oxygen and carbon dioxide in the blood as well as manufacture of haemoglobin hence higher values indicate a greater potential for this function and a better state of health [20].

In Figure 5 (3), the results showed that normal blood hemoglobin (g/dl) was noted in group A (control) and in treated groups (13.27, 14.90, 15.14 and 13.94 g/dl), respectively. In treated group C the maximum hemoglobin was counted (15.14 g/dl). In control group A average hemoglobin was counted (13.27 g/dl). Statistically analysis that there were in two groups control group A and group D, where the means were not significantly different from one another while significant from groups B and D, respectively. In group C the results was shows that highest hemoglobin counted than other treated and control groups, respectively.

In Figure 5 (4), the result are showed the average packed cells volume in control and treated groups were counted (30.73, 32.94, 33.73 and 33.44%) respectively, Maximum packed cell volume was in group C (33.73%), minimum packed cell volume counted level (30.73%) was counted in control group (A), where the broiler chicks did not supplied phyase enzyme. Statistically analysis there were in three groups (B, C and D) the means were not significantly different from one another, while significant from group A (control), respectively. The PCV values obtained in this study though it differed significantly among the group was within normal range [21]; the values obtained for all the treatment groups indicate nutritional adequacy of all diets and presence of a toxic factor, since values did not indicate malor under nutrition [22]. PCV is an index of toxicity reduction in the blood usually and suggest presence of a toxic factor which has adverse effect on blood formation [23].

In Figure 5 (5), the result showed that the average of blood glucose counted in A (control) and treatment groups (12.43, 10.85, 10.12, and 11.26 mmol/L), respectively. In group A (control) the maximum blood glucose level was counted (12.43 mmol/L), minimum blood glucose count was noted (10.12 mmol/L) in treated group C, respectively. There were two groups (B and C) in which the means were not significantly different from one another while significant from group A and D, respectively.

In Figure 5 (6), the result showed that the average of total proteins count in treated groups and control (A) group was (5.43, 5.82, 5.88 and 5.47 mg/dl), respectively. In treated group C the maximum total proteins count was noted, as related to others groups. Minimum total protein count level (5.43) was reported in control (A) group, where the chicks were not provided phyase enzyme. Statistical analysis showed that, there were non-significantly different from one another, respectively.