Research on the production of biodiesel from lignocellulosic biomass started in 2007, Figure 1 shows the number of articles published per year, on biodiesel production from lignocellulosic biomass (production of lignocellulosic biodiesel), compared to the number of articles on first-generation biodiesel production (biodiesel produced from feedstock like grains and seed oils), from 1993 to 2019. Articles on first-generation biodiesel production used the word “biodiesel”, and they were retrieved from the Web of Science database. The Articles on lignocellulosic biodiesel production were taken from the Scopus, Compendex, and Web of Science databases. The first publication on first-generation biodiesel dates from 1993; 14 years the first publications on lignocellulosic biodiesel come out.

There was a significant increase in the publication of articles on first-generation biodiesel production in the 2000s, showing great progress particularly in the development of biodiesel technology and the economic sustainability of small and large biodiesel production plants [22]. This growth coincided with a marked increase in oil and diesel prices, which boosted the competitiveness of biodiesel industry. Biofuels are the renewable energy sources most closely linked to the price of oil; other renewable energy sources, such as wind, geothermal, and solar, are less sensitive to this factor [23] [24]. The production of articles on lignocel

lulosic biodiesel has fluctuated in recent years in response to investment policies, which directly affect the publication of articles. Global pressures due to environmental problems have led some governments to stimulate grater production capacity, establishing minimum levels of biodiesel in diesel-biodiesel blends [25] [26]. There is also competition from other renewables, like wind and solar power, which also receive government incentives and have been the focus of attention in recent years, leaving biofuels in the second line [1] [27]. Another key factor is the conflict of interests between the regulatory state and agro-industry, which is marked by some specific institutional and economic conditions and can lead to favorable or unfavorable scenarios, depending on the circumstances [28].

The contribution of research by countries and by entities, can be estimated according to the corresponding address of the author. Table 1 shows the production of scientific articles by country and region. Asia was the continent with the highest production of articles, almost doubled the production of America. China was the largest publisher of scientific articles, according to the International Energy Agency, energy consumption in China has increased considerably due to rapid economic expansion, [29] resulting in a marked increase in demand for oil imports, [30] in response, China established an ambitious biodiesel research goals as part of the renewable energy initiative [31]. USA is the world´s leading producer of first-generation biodiesel in the world, however it is the second researcher on lignocellulosic biodiesel. Third with 17 articles is India, a country that has made significant investments in renewable energies in recent years, driven

by economic progress, growth in domestic consumption and increased demand for energy, especially in the transport sector [32]. According to the World Economic Forum, China, USA and India, will drive the global expansion of renewables [33]. Publication of articles in Europe is more evenly distributed between the countries, while western European countries are more productive. Finally, in Africa and Oceania only three articles were published each. Members of the Group of Seven, or G7 (Canada, France, Germany, Italy, Japan, UK, and USA), are among the 15 top publishers of articles, accounting for 36.84%. This is probably a consequence of earliest studies of first-generation biodiesel carried out in these more advanced economies, which yielded the most significant developments [34].

International collaboration is important for research and innovation in renewable energies, because it can lead to higher-quality inventions at a lower cost, as resources and investments are shared and, according to recent evidence, the inventions are generated at a faster pace [35] [36]. The International Energy Agency recommends expanding international collaborative energy R & D efforts to reduce costs and improve investment efficiency [37]. Figure 2 shows the cross-border cooperation in research into lignocellulosic biodiesel production. The size of the circle for each country is an approximate representation of the number of articles published, each line represents one article published per association.

Countries with technological affinities tend to collaborate [36], so the distribution of the collaboration network indicates the position of technological capacity of each country compare to others. Forty international partnerships on lignocellulosic biodiesel research account for 15%, top collaborators countries are USA, Germany, and China, which is similar to the international collaboration pattern observed in research on solar and wind energy research, between USA, China, and the European Union [1]. In the lower right corner of Figure 2, there is also an isolated group of collaborators: Ireland, Greece, and Norway.

China-USA is the most prolific collaboration, resulting in nine articles. The world’s leading investors in energy research and with similar technological potential, also tend to collaborate in other fields of science [1] [38]; four out of nine collaborations China-USA were between universities, three were between a university and a research institute, and two were between enterprises. Six partnerships focus on conversion methods using lipid-producing microorganisms. USA is in the middle of the research collaborations network with 22 collaborations, followed by China (16), and Germany (11). India, the third researcher country, has been involved in only two international partnerships; France, with six articles, has not collaborated internationally. Top researcher countries on first-generation biodiesel, are USA, China and Brazil, however, Brazil falls to tenth place in research on lignocellulosic biodiesel production. Similarly, Brazil worked in multiple international cooperation initiatives on first-generation biodiesel, but has only partnered with the USA and Sweden in research on lignocellulosic biodiesel [39]. The primary interest of US-China partnerships research on lignocellulosic biodiesel production was the study of lipid-producing microorganisms, with 6 out of 9 collaborations [40] - [45].

Figure 3 shows the top 10 research countries on lignocellulosic biodiesel, which together represent 212 articles (80%). Research on the production of lignocellulosic biodiesel, resulted in 260 articles, the universities are responsible for most of the publications (205) and then the institutes (49), only six articles were produced by enterprises. China is the main research country, considering universities and institutions, the Dalian Institute of Chemical Physics (DICP) is the main publisher with 12 articles, followed by the South China University of Technology

with 11. The DICP focuses on sustainable energy research, environmental optimization, biotechnology, and advanced materials. Zongbao Zhao and collaborators from the DICP are the author of 6 publications, their research focuses on the biotransformation of lignocellulosic biomass into microbial oil and other fine chemicals. Also from DICP, Tao Zhang and collaborators with 4 articles, focuses on the use of catalysts. Minhua Zong and collaborators with 5 publications and Hong Wu and collaborators with 4, from the South China University of Technology, have studied lipid-producing microorganisms. Huazhong Agricultural University and Tsinghua University are other productive universities in China, with 5 publications each.

USA is the second most important research country by universities and institutions (64 articles), the Massachusetts Institute of Technology (MIT), the University of California, Berkeley, and Washington State University published 6 articles each. Shulin Chen and collaborators from Washington State University, are the author of 5 articles, their research is oriented to the study of lipid-producing microorganisms, and to the study of enzymes from the digestive system of termites [46]. Anthony Sinskey and collaborators from MIT, focusing on the genetic modification of bacteria to produce fatty acids, used Rhodococcus opacus. Wei Liao and collaborators from Michigan State University have studied integrated systems for the effective use of municipal and agricultural waste.

Articles with a high number of citations tend to indicate new frontiers in their specific field [47]. Table 2 shows the ten most cited articles, five of them are related to the microbial lipid production, indicating that this conversion method is the most interesting in the scientific community. “Microbial production of fatty-acid-derived fuels and chemicals from plant biomass,” by Jay Keasling from the University of California Berkeley, is the most cited article. Published in 2010 in Nature, it discusses the production of fatty esters by modified Escherichia coli [48]. Biofuels with linear hydrocarbon chains have been found to freeze more easily at low temperatures [49], the Keasling Lab is working on the production of

Source: Based on data from the Web of Science. Number of citations as reported in June 2020.

more branched and cyclic hydrocarbons, using a modified Escherichia coli and Saccharomyces cerevisiae [50].

The second most cited article is “Selective hydrolysis of cellulose into glucose over solid acid catalysts,” by Onda, et al. (2008) at Kochi University, Japan. Published in Green Chemistry, it focuses on the hydrolysis of cellulose [51]. Onda et al. (2009) also studied on the acid hydrolysis of glucose under hydrothermal conditions, this study ranks 24th in number of citations, with 122 in total [52]. The third most cited article, is “High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture,” by Zhao, et al. (2007), from Dalian University of Technology [53]. Zhao and collaborators wrote two highly cited articles, in position 18 with 152 citations, a research on the effects of some byproducts generated the hydrolysis of lignocellulosic biomass on fermentation with Rhodosporidium toruloides Y4 [54], and in position 19 with 141 citations, an article on medium optimization for the co-fermentation of glucose and xylose with Lipomyces starkeyi [55].

Three hundred scientific articles on the production of lignocellulosic biodiesel, were published in 97 different journals. Table 3 shows the journals with more articles published on lignocellulosic biodiesel production, the top five journals together represent 38% of all the articles. The first leading journal Bioresource technology, covers all areas relating to the treatment of biological waste, biomass, bioenergy, biotransformation, and technologies associated with conversion or production.

Biotechnology for Biofuels, the second top publisher, addresses renewable feedstocks and technological and operational progress in biofuel production. It also covers the economic, environmental, and institutional considerations relating to biofuel production. Finally, Green Chemistry, focuses on the environmental impact of chemicals and fuels, and the development of alternatives and sustainable technologies that are not toxic to living beings and the environment.

Source: Based on data from the Web of Science, Compendex, and Scopus databases. Impact factor according to the Journal Citation Reports, 2019.

The journals with the highest impact factors are Nature (1 article), whose impact factor is 42.778 (2019 Journal Citation Reports), and Energy & Environmental Science (4 articles), with an impact factor of 30,289 (2019 Journal Citation Reports).

Researchers often choose a few keywords to highlight the central idea of each publication. Author keywords have recently been used to analyze trends and identify key research points [56]. Here, we analyze keywords and publication titles to find critical aspects of the studies. Table 4 shows the 20 keywords most commonly selected by the authors of articles on lignocellulosic biodiesel production. The first keyword, “biodiesel” was the most used and some frequent combinations were “biodiesel production” and “biodiesel fuel”. In contrast, the keywords “synthetic diesel” and “renewable diesel” appear relatively infrequently. The second most common keyword was “biomass”, it had a lot of combinations like “lignocellulosic biomass”, “biomass conversion”, “biomass hydrolyzate”, “biomass pretreatment”, “biomass pyrolysis” and “biomass-derived”. The term “lignocellulosic”, can be combined with other keywords such as “lignocellulosic hydrolysate”, “lignocellulosic material”, and “lignocellulosic sugar”, these terms are frequently used in biomass pretreatment. The fifth keyword “hydrolyzate” indicates that the most widely used pretreatment for lignocellulosic biomass is hydrolysis. The tenth keyword “pretreatment” appears with many combinations, as a result of the variety of pretreatments such as “acid pretreatment”, “alkaline pretreatment”, “enzymatic pretreatment” and “thermochemical pretreatment”.

The seventh keyword “microbial”, indicates that the most widely used technique for converting lignocellulosic biomass into biodiesel is microbial lipid production. The 12th keyword “pyrolysis” have been examined last decade in different forms of pyrolysis, such as catalytic pyrolysis and fast pyrolysis. The 8th

Source: Based on data from the Web of Science, Compendex, and Scopus databases.

keyword “yeast” suggests that this microorganism is the most used for the conversion of lignocellulosic biomass, the specific yeasts mentioned are: “Cryptococcus” in position 13, “Trichosporon” in position 14, “Thodosporidium” in position 17, and “Rhodotorula” in position 20. The bacterium “Rhodococcus” appears in position 19. The lignocellulosic biomasses most used are “corn” in position 11, and “rice” in position 15. Other keywords used were “bagasse” in position 16, which is frequently combined with “sugarcane” and “sorghum”. “Straw” at position 18 is another common keyword, specifically used “rice straw”, “wheat straw” and “canola straw”.

To identify patent applications, the Derwent Innovation database was used, which provides access to most of the world’s patent offices. The patent applications were taken from the DWPI Manual codes: H06-B04A (biodiesel compounds), Q51-D03 (diesel compounds), and X22-A20C (diesel compounds), these codes were the result of searching with the world “biodiesel”. These codes were combined with the keywords “lignocellulosic”, “lignocellulose”, “cellulosic” and “cellulose”. This search produced 563 patent applications, after reading the abstracts were retrieved 169 relevant patent applications, i.e. patent applications focus on the production of biodiesel from lignocellulosic biomass. The search was done in June 2020, so any patent document on lignocellulosic biodiesel production processes added to the database after this date were not retrieved here.

The most representative IPC (International Patent Classifications) classes and subclasses were taken from the IPC codes of the 169 relevant patent applications. These IPC subclasses were identified to assist with future use in Industrial property monitoring. Table 5 shows the IPC classes and subclasses that appeared frequently in the patent applications on the production of lignocellulosic biodiesel.

The recovered IPC codes, classified into three main IPC classes: C10L—additives for biodiesel; C10G—mixtures of biodiesel and other fuels; and C12P—biochemical processes (fermentation processes or processes that use enzymes) for biodiesel production. Classes C12P-7 (oxygen-containing organic compounds) are the most frequent, followed by C10L-1 (liquid carbonaceous fuels) and C10G-1 (production of liquid hydrocarbon mixtures from oil shale, oil-sand, or non-melting solid carbonaceous or similar materials, e.g. wood). The most frequent subclasses related to the product (C12P-7/64, C12P-7/00, C10L-1/02, C10L-1/00, C10L-1/18, C10G-1/00, C10G-3/00), the type of biomass (C12P-7/10, C12P-19/02), or the type of conversion or reagents used (C12P-7/14, C10G-1/06, C10G-1/08, C12P-19/14), are important in the future monitoring of lignocellulosic biodiesel.

Figure 4 shows the global production in patent applications on lignocellulosic biodiesel production by year. The first’s two patent applications were filed in

Source: Based on data from Derwent innovation.

2005, one in China (CN1624076-A) describes equipment using acid hydrolysis of lignocellulose biomass, the second was filed in USA (US2005177013-A1) and describes the conversion of agro-industrial waste into biofuels, such as crude oil, diesel, gasoline, and other fuels or oil products. There was an upward trend in patent applications until 2013, after which a decrease can be observed. An analysis of the global biofuel patent scenario shows that since the boom in the early 2000s, inventions in biofuel technologies have declined considerably and in many countries have gone into decline. Global trends indicate an uncertain future, especially for advanced biofuels [25]. It should be remembered that there is an 18-month secrecy period after a patent application is filed before it is indexed on databases. Therefore, the 2018 and 2019 data in Figure 4, do not necessarily indicate an actual reduction in patents.

Figure 5 shows the top ten countries in patent applications on lignocellulosic biodiesel production. USA and China together, account for 60% of the global patenting application. Sixty-nine out of 84 USA patent applications were filed by enterprises, while only six were filed by individuals and four by universities. Although China is highly productive on research (96 scientific articles), its patenting activity is relatively low (25 patent applications). Fifteen out of 25 Chinese patent applications were filed by Universities, six were by research institutes, and just one was an individual and one an enterprise. This limited patenting activity by enterprises could be due to the low biodiesel production levels in China because of a limited supply of raw materials [57]. There are over 50 biodiesel producer enterprises in China, most with a production capacity of fewer than 50.000 tons/year. Private biodiesel producer Chinese businesses account for over 95% of overall biodiesel production. Almost all biodiesel in China comes from used oil,

thanks to national political guidelines and incentives. India was the third top researcher, but no patent applications filed were identified.

Enterprises are responsible for the majority of patent applications filed (108 patent applications, 67%), followed by universities (15%), research institutes (13%) and individuals (5%). Only 8 patent applications (15%) were submitted by partnerships. Four of these partnerships were between enterprises (US2009064567-A1, WO2010063031-A2, WO2009059936-A2, WO2009152873-A1), three between an enterprise and a research institute (JP2014139283-A, WO2015193588-A1, WO2012030215-A1), and one between an enterprise and a university (US2010279372-A1). Table 6 shows the names and codes of entities with more than two patent applications, this table shows how diverse the global players are, in the development of lignocellulosic biodiesel innovations, ranging from enterprises on biotechnology field (Xyleco Inc.), oil and energy (BP Corp. North America Inc., Shell Oil Co., Eni S.P.A., Skandinavisk Kemiinformation), and biofuels (Kior Inc., Cool Planet Energy Systems Inc.). Shell is the enterprise with more patent applications filed (11), Six of these (US2012317873-A1, US2013152456-A1, WO2013066808-A1, US2013109896-A1, US2014165456-A1, WO2017004001-A1) describe hydrothermal and hydrocatalytic treatments for the conversion from lignocellulosic biomass to synthetic gasoline, diesel, jet-fuel, and other chemical compounds, with different variations, such as: use of catalytic hydrogenolysis with sulfur, molybdenum, and tungsten; a solid-separation filter, a hydrothermal digestion unit and a fluid circulation circuit; hydrogenation, hydrodeoxygenation, and liquid-liquid separation; catalytic hydrogenolysis using cobalt and nickel with aluminum support and high-pressure hydrocatalytic treatment. Two Shell patents applications (US2012152836-A1, US2016298292-A1)

Source: Based on data from Derwent innovation. Period of analysis: 1996-2019.

describe the selective removal of compounds, with variations such as: removal of sulfur compounds and nitrogen compounds with hydrogenation and catalytic hydrogenolysis; selective removal of calcium, potassium, magnesium, and transition metals, followed by hydrothermal treatment. Finally, three Shell patent applications (US2013152457-A1, WO2013074437-A1, US2014096438-A1) use different techniques, including pressurization and digestion, catalytic hydropyrolysis, and biomass liquefaction.

Neste Oil has filed six patent applications (WO2010149859-A2, EP2468875-A1, EP2468877-A1, WO2012085340-A1, EP2735610-A1, WO2015086780-A1), these describe the use of lipid-producing microorganisms. They include genetically modified oleaginous bacteria to increase their lipid production; lipid-producing microorganisms in liquid-liquid and liquid-solid media; improvements in the enzyme stabilization system, filtration and separation methods, and tolerance to some inhibitors. BP Corp. North America has filed five patent applications (US2010146844-A1, WO2010129101-A1, US2010331560-A1, WO2013165962-A2, US2013130335-A1), aimed at producing synthetic gasoline, biodiesel, and chemicals. Major innovations include: depolymerization and extraction of pentoses; recycling of hydrolytic enzymes; removal of lignin; separation of pretreated biomass into separated fractions with high concentrations of lignin and sugars; and fermentation. The Swedish Skandanavisk Kemiinformation AB with four patent applications (SE201300378-A1, WO2013095204-A1, SE201000690-A1, SE201600009-A1), describe processes such as: lignin removal using solvents followed by catalytic depolymerization; hydrolysis and fermentation; catalytic pyrolysis and separation of cellulose from lignin by sodium hydroxide. Kior Inc. with four patent applications (WO2009118352-A1, US2012101318-A1, US2012204481-A1, US2012232299-A1) described bio-oil upgrading by inorganic catalysis and biocatalytic cracking, deoxygenation, hydrotreatment, fractioning of bio-oil and catalytic thermolysis of biomass.

Wuhan University of Science and Technology was the university with more patent applications filed (CN106755148-A, CN102660312-A, CN102154381-A), these documents described processes such as: fermentation controlled by carbon and nitrogen levels, oil extraction using solvent of organic and the use of enzymatic degradation. Two other Chinese universities have filed two patent applications: Huazhong University (CN103923948-A, CN101591684-A), and Zhejiang University (CN101255348-A, CN1624076-A). China is also the lider on patent applications filed by research institutes (6), followed by USA (5) and France (5). The Institut Français du Pétrole is the institute with more patent applications filed (FR2921385-A1, FR2957607-A1, WO2015193588-A1).