Three check varieties IR64, Black Gora and Azucena were included with eighteen accessions collected from Bangladesh Rice Research Institute (BRRI) for screening as follows:

1) Aus cultivars—BR 21, BRRI 42, BRRI 48.

2) Aman cultivars—BRRI 32, BRRI 30, BRRI 49, BRRI 51, BRRI 52, BR 22.

3) Boro cultivars—BR 19, BRRI 29, BRRI 50, BRRI 28, BR 3, BRRI 36, BR 14, BR 16, BRRI 60.

The experiment was set up on 14 December 2018. Four replicates including three check varieties and thirteen accessions were organized as a randomized complete block design. The all 16 accessions were tested in the hydroponic set up. Azucena, Black Gora and IR 64 were included with those accessions as check varieties.

Seeds were first surface sterilized by soaking with five times diluted domestic bleach 1% sodium chloro hypochlorite (NaHClO3) for approximately 5 minutes and washed three times with tap water. Seeds were then germinated at 30˚C for three days. Germinated seedlings were transferred in black seedling trays containing 84 wells (12 × 7 wells) with 55 mm deep and 12 mm diameter at the bottom and 40 mm on the top. Germinated seedlings were placed on the piece of a mesh (15 × 5 mm) resting above the hole of a well. Each seedling tray was fitted in the 50 L plastic box 56 cm long, 36 cm wide and 29 cm deep. The empty wells were covered by the aluminum foil to prevent the entry of the light to the nutrient solution while the sides of the boxes were covered by plastic sheets that was white on the exterior and black on the interior. Plastic sheets were used to prevent algae formation upon light penetration. Aeration system was set up in the box. Each box filled with 50 L half strength Yoshida’s nutrient solution (pH5.5) [26] for the first three weeks then full-strength nutrient solution for the final four weeks. Nutrient solutions were replaced twice a week and pH was adjusted daily. The experiment was conducted in the Department of Biochemistry and molecular Biology laboratory, University of Chittagong, humidity was maintained 70% and the temperature was 25˚C to 28˚C throughout the experiment. The supplementary lights were supplied 120 μMm-2s-1 (PAR) 12 hrs. a day. The maximum root and shoot length of each plant were recorded weekly for five weeks (Figure 1).

Two check varieties IR64 and Azucena were included with six accessions collected from Bangladesh Rice Research Institute (BRRI)—BR 3 (Boro), BR 14 (Boro), BR 19 (Boro), BRRI 60 (Boro), BR 22 (Aman), BRRI 32 (Aman) for screening.

1) Sheet of 4 mm thick glass cut to 1200 mm × 300 mm.

2) Sandy loam soil sieved using a coarse sieve (approximately 5 mm mesh) to remove stone and large clumps.

3) Drip irrigation system (available in gardening stores).

4) Supplies using duct tape, two lengths of 15 mm thick and 100 mm long wood, bottle tops custom made metal chute to guide soil and chemicals for nutrient solution.

The experiment was set up on 8 April 2019. Four replicates including two check varieties and six accessions were organized as a randomized complete block design and set for rhizotron screening. Plants were grown for 42 days and water was withheld from day 39 so that daily water use in the last 3 days could be assessed. Two glass sheets are selected, at least one of which clean on both sides. One is placed on a work surface with two of four edges slightly overhanging. Two lengths of 15 mm thick wood are placed on the top of the first sheet, a 15 mm bottle top is placed at the top and the bottom of the glass as spacers and the second sheet of glass were placed over the top. Duct tape is used to join the two sheets of glass together at the overhanging edges. The sheets are turned so that remaining long edges are overhanging and that is then sealed with duct tape. Three of the four sides are therefore completely sealed with duct tape. The empty rhizotron are set vertical, a single strip of duct tape is wound right around at a depth of about 300 mm from the bottom, and two length of wood are removed. The bottle tops prevent the glass from together and empty rhizotron can therefore be stacked and waiting for filling. The bottle top can be removed at a later date during the soil filling process. The bottle top at the bottom must remain in the rhizotron. The empty rhizotron is stood upon a soft support such as expanded polystyrene sheet and sieved soil is then encouraged into the rhizotron using the dustpan and custom-made guide. When the rhizotron is nearly full, the upper bottle top can be removed. When full, the rhizotron is lifted and then gently dropped onto the support, causing the soil level to drop 10 - 15 cm due to packing of the soil. The rhizotron is refilled, gently dropped once more and refilled to within 5 mm of the top for a final time. The force of the drop will affect the amount of soil used. The aim is to pack the soil sufficiently well to prevent slumping of the soil when it is watered but not so much that it splits the tape, or create impedance to the roots. The latter can be roughly assessed by pushing a sharpened pencil into the soil. If it is difficult to push, the roots will probably also find it difficult. Once filled the rhizotron should be weighed with aim to have each rhizotron the same weight. Typically, the rhizotrons weigh 13 kg and contain about 7 kg of wet soil. A small drainage hole should be made at each side at the bottom using an implement such as a sharpened pencil. Typically, rhizotrons are placed in stacks of eight and are learned at an angle of 15˚ to encourage roots to grow on the lower face. The exposed face of the first stack was backed with an insulation sheet to reduce heat exchange and prevent light penetration. Insulation was placed over the front of the stacks and an irrigation system was installed. This should supply water equally to each rhizotron and apply it slowly (to avoid soil clumping). Typically, an irrigation rate of 40 ml/min is used. This can be used to supply nutrients and water as required.

Each rhizotron is labeled on the lower sheet so that in a photograph of that side (where roots are most visible) the identity of the rhizotron can be seen. Typically, two seeds were sown on each rhizotron, and thinned to one when they have emerged. Watering was typically done three times a week with 250 ml of Yoshida’s nutrient solution for the first 3 weeks, moving up to more frequent and larger volumes of nutrient and water as the plant grow reaching about 250 ml nutrient and 150 ml of water every day when 6 weeks old. However, amounts will vary with the environment. Photograph of the lower side of each rhizotron can be taken when must be done in the dark to reduce reflection from the glass. Those can be done at night using spotlights at either side of the rhizotrons. On a weekly basis, shoot growth was monitored as height of the plant (length from the soil to the top of the longest leaf) while the length of the longest visible root and the number of the root that were passed 25, 50, 75 and 100 cm are recorded (Figure 2).

Collected root and shoot traits data from hydroponic and rhizotron experiment, were analyzed by using Minitab version 17. The data was first transformed to make the data normally distributed, then corrected for block effects and finally differences between cultivars assessed using ANOVA.

The mean root length and shoot length increased in a linear fashion with time (Figure 3). Plants had a mean root length of 1.775 cm at day 26 which increased up to 26.025 cm at day 52 and had a mean shoot length of 16.52 cm at day 26 which increased up to 29.72 cm at day 52. Shoot growth showed a greater increase from 7.85 cm at days 26 to 41.35 cm at day 52. A total of 16 cultivars tested in the hydroponic experiment showed a great difference in root length and shoot length throughout the experiment. The maximum root length and shoot length of all genotypes at days 52 is shown in Figures 3-5.

The check variety Azucena had the highest root length while BRRI 32 Aman cultivar had the lowest root length at day 26 (Figure 6). Again, BR 3 cultivar had the highest root length while BRRI 48 Aus cultivar had the lowest root length at day 26 (Figure 7).

At Day 52, BRRI 60 Boro cultivar had the highest root length while BRRI 48 Aus cultivar had the lowest root length (Figure 8).

In case of shoot length analysis, Black Gora had the highest shoot length in cm at Day 26 while BRRI 36 Boro cultivar had the lowest shoot length in cm (Figure 9) At day 37, Black Gora had the highest shoot length and BRRI 48 Aus had the lowest shoot length (Figure 9) At day 52, these two again showed the highest and the lowest shoot lengths respectively (Figure 10, Figure 11).

In case of root length analysis, significant variations were observed at all days of measurement and the proportion of variations explained by the genotypes were increased from day 26 to day 52 where it was highest at day 37. That means the

highest discrimination of root length was observed at day 37. Azucena, Black Gora, BR 3, BR 19, BR 16, BRRI 60 had the longest root on that day while IR64, BRRI 51, BRRI 36, had the shortest.

The maximum root length of days 37 ranges from 14 cm to16 cm and Black Gora and BR3 were the highest and BRRI 36 and IR 64 were the lowest and at that time 58.27% variation explained by the genotype (P < 0.001).

The data of root length and shoot length were recorded at 52 days after sowing (DAS) of hydroponic experiment having significant result (P < 0.001). Black Gora, BRRI 60 and BR 3 had longest root length and IR 64, BRRI 51 and BRRI 36 had the shortest root length.

In the root length and shoot length data for three control varieties Azucena, Black Gora and IR64 (check varieties), significant variation was observed from day 5 to day 52 (DAS) and the proportion of variation explained by the genotypes were increased from day 5 to 52 (Figure 12, Figure 13).

In rhizotron screening method, the mean root length and shoot length in soil filled rhizotron increased in a linear fashion with time (Figure 14).

The maximum visible root length along with the shoot length (cm) has shown in the Figures 14-16. In the same time maximum visible root and shoot length

data has shown individually in the Figures 17-22 and the check varieties in the Figure 23, Figure 24.

The maximum visible root length of the check varieties shows that Azucena had long with vertical axes and IR 64 had shallow rooted which follows the same pattern as previously detected data [27].

The maximum visible root length measured in the rhizotron experiment increased 110 cm at day 46 from 66 cm at day 24. The proportion variation explained by genotypes for the maximum visible root length showed an increased pattern from day 24 to day 46 respectively. Among the landraces genotype BRRI 60, BR19 and BR3 had the longest visible root which were higher at all stages than the mean root length BR14 and BR32 had the shortest root systems (Figure 14).

One way analysis of variance (ANOVA) revealed 73.37% variation (P < 0.001) explained by the genotype at 46 days after sowing (DAS) and three BBRI cultivars had the highest root length and two showed the shallow rooted cultivars.

When comparing the hydroponic root traits data with rhizotron, at least three BRRI cultivars BRRI 60 (Boro), BR 22 (Aman) and BR 3 (Boro) showed deep rooted cultivars in both soil (rhizotron) and liquid media(hydroponic).

Hydroponic screens have the advantage that the roots can be continually monitored for root growth, however after a period of time the roots become intertwine each other and the pH reduces rapidly [11]. Also, hydroponics is a non-soil based system, so if the roots respond in a solution system to how they would in a soil system could be an issue. Another screening method is rhizotrons, the main advantages are that the plants are grown in soil and that root angle information can be assessed. In addition stresses related to nutrients or water can be measured. Like hydroponics this system can be continually measured, however due to their 2D construction they do limit root growth in all natural directions as well as being a labour intensive screen, therefore reducing their use in high throughput screens.

Initially 16 accessions were assessed in hydroponic experiment. Significant variation was observed between genotypes which reflected the variation of rooting behavior of the genotypes. In rhizotron experiment, six cultivars along with two check varieties were screened. The deep rooted cultivars detected from the hydroponic experiment as for example, BRRI 32 and BRRI 60, also revealed the deep rooted as well in rhizotron experiment. Comparing the result of hydroponic and rhizotron methods BRRI 32 and BRRI 60 was surprising since it has already been shown deep rooted cultivars in different planting methods. This data already been reported in BRRI annual report 2015-16 [28]. Comparing the result of the other methods, hydroponic and rhizotron screening methods has given confidence for the tested of the cultivars.