The domestication of forest trees as new crops is now recognized for their ability to transform tropical land used by improving agricultural production/food security; increasing farmers’ income and their well-being [1] [2]. Garcinia kola belongs to the Clusiaceae family and it’s endemic in the humid lowland rainforest vegetation of the West and Central African [3]. Like many other forest trees, G. kola is produced traditionally and constitutes an important commercial product [4] [5]. For example, its fruits, edible nuts and roots have medicinal importance and its branches with bactericidal properties, are used as chewing sticks [6]. The seed contains a chemical called kolaviron, a biflavonoïdes which has antimalarial properties [7]. Seed extracts are also used in the treatment of diarrhoea [8], bronchitis and throat infections [9] and anti-sickling activities [10].

In Côte d’Ivoire, seeds are recently used in breweries industries to make soda or energy drink. The species has a high local market demand or export [11] [12]. These several demands increase the level of the species exploitation. But its overexploitation is currently done on the natural population of species. This situation coupled with deforestation, limits seriously the natural regeneration of the species [13]. Several studies showed that G. Kola has a threatened status in central and west Africa tropical forest [12] [14] [15].

G. kola can only regenerate from seed (kernel) in natural environments. Unfortunately, the low germination of its seeds has been noticed as a major problem in natural regeneration or for huge quantity of seedlings production, due to their high level of dormancy [3] [11] [16]. To promote the cultivation of G. kola, a lot of works have been led to investigate seed germination [3] [11] [16] [17]. Different methods were used for the seed’s dormancy breaking, such as stratification [18] and soaking in Gibberellic acid solution [11] to obtain over than 96% germination. However, these technologies are not available to farmers. Also, reproduction by seeds (sexual reproduction) still does not allow individuals (hybrids) identical to the mother plant to be obtained. In fact, this hybrid plant can have different characters from those of the mother plant [19].

Another problem is seeds availability to use it for seedlings production, since the seeds are commonly used for local and international trade.

Besides, few experiments were also conducted on vegetative propagation to investigate grafting [20] and juvenile stem cuttings [21]. Unlike plants of the Aceraceae family (Palm), almost all plants can reproduce by macro-cuttings due to the presence of axillary buds on twigs and stems [22] [23]. Indeed, vegetative propagation by cuttings is a technique of conform reproduction of mother plant, unlike sexual reproduction. In addition, this technique can induce an early fruiting of plants [24].

Difficult rooting ability of stem cuttings collected from branches of mature trees of G. kola was reported by [21]. Other Garcinia species (Garcinia pedunculata Roxb. ex Buch.-Ham. and Garcinia morella (Gaertn.) Desr.) were successful propagated by using juvenile stem cutting from seedlings [25]. But little information was given about the role of leaf and cutting physiological state on rooting of stem cuttings of Garcinia. kola. However, many indigenous trees species were propagated by leafy stem cutting [24]. According to several authors, successful propagation of leafy stem cuttings depends on many factors and they interactions such as plant species, rooting media, leaf area, cutting physiological state and propagation environment [24] [26] [27] [28]. In addition, most studies investigated on rooting ability of stem cutting position. In case of G. kola, softwood cuttings presented high rooting ability [21]. For other trees such as Dalbergia sissoo [29] and Dalbergia melanoxylon [30] the best rooting ability were recorded on cuttings from basal and middle position than those from apical position of stock shoot. Effect of leaf on rooting ability of stem cuttings has been reported in many trees [31] [32] and noted that photosynthesis activities depend of the optimum leaf area.

In a survey of farmer’s species for agroforestry, G. kola was identified as one of the top priority species for domestication in the humid lowlands of West Africa [33]. To domesticate species like G. kola as new crops, vegetative propagation protocols have been developed [24]. So, these protocols make possible to multiply and select the best seedlings and used them as horticultural cultivars. Thus, [34] have developed non-mist poly-propagators for the rooting of stem cuttings for the use of farmers in remote rural areas when domesticating indigenous fruit and nut trees. This rapid clonal multiplication approach to domestication is considered ideal for the rapid multiplication of a species under threat and also allows the propagation of selected trees with superior characteristics. In G. kola, vegetative propagation studies have focussed on stem and root cuttings [21]. These studies have indicated that the greatest success is achieved when cuttings are treated with 2.5 g/l of indole butyric acid (IBA). To promote the domestication of G. kola in agroforestry, it will be necessary to further develop efficient methods and techniques of clonally propagating G. kola which are easily transferable to farmers and which are affordable given the cost of phytohormones.

The present study, done under non-mist poly-propagator system, fits in this context. The objective of study was to develop vegetative propagation methods testing the hypothesis that successful rooting and sprouting of leafy stem cuttings can be achieved by optimising the level of stem lignification and the lamina area of the leaf.

The different cuttings types from G. kola seedlings presented good ability on rooting and shooting. Rooting percentage increases from greenwood to hardwood cutting. The best rooting percentage and mean primary root number per cutting observed with hardwood cuttings is probably due to the high-level content of carbohydrates or food reserves [35]. However, these results with G. kola differ to some extent from the generally expected norm in which cuttings from the top of the shoot have an inherently greater rooting ability [24]. Unlike the situation in many other species, this can be explained to some extent by the relatively constant cutting length in the present study whereas many other species like Triplochiton scleroxylon K. Schum. which have longer internodes at top of their shoots [36]. Generally, longer stem cuttings from juvenile shoots have greater capacity for rooting. This can be explained by their greater storage capacity for current assimilates in the propagator before the emergence of new roots [30]. [36] also found some evidence that storage capacity was determined by stem thickness (cutting diameter). In the present study, the successful rooting of hardwood cutting can be due to their large diameter. Similar results were noticed by [37] on vegetative propagation of Ficus roxburghii Wall. These authors noticed that cuttings with large diameter presented higher sprouting and rooting ability due to their more food reserves in these cuttings. According to [38], the sprouting of cutting depends on food reserves available within the cuttings in vegetative propagation.

Regarding shooting percentage, mean shoot number per cutting, mean shoot length and mean leaf number per cutting, the best value observed to greenwood cutting is due to it physiological state. Three or two shoots sprouts on the apical part of the cutting. While, one or two axillaries buds’ sprouts on the other cutting type.

Rooting and shooting percentage, mean primary root length, mean secondary root number and mean shoot length enhance with increasing of leaf area. The best values observed to cutting with 113 cm² leaf area followed by those of 84.75 cm² leaf area, can be explained by the higher photosynthesis activities of these cuttings which produce carbohydrates and provide a source of energy essential for root emission [39]. The results of the present study with G. kola are conform to existing reports on the rooting of stem cuttings in other tropical tree species, such as: Triplochiton scleroxylon [26], Cordia alliodora (Ruiz & Pavon) [40], Lovoa trichilioides Harms [41] in terms of the very important role of the leaf on cutting propagation. But the results of G. kola differ of those of Shorea leprosula Roxb. ex C.F. Gaerth which stem cuttings rooting decrease with increasing of leaf area [42].

Combined effect of cutting type and leaf area significantly influenced all the rooting and shooting parameters. The best rooting and shooting percentage of cutting with 113 cm² leaf area followed by those of 84.75 cm² and 56.5 cm² leaf area whatever cutting type can be explained by the high level of photosynthesis activities which produce important quantities of carbohydrates for root formation and elongation. Investigations on the role of the leaf in the rooting process have indicated that rooting ability is maximised when severed cuttings are photosynthetically active and producing assimilates for root development and elongation of the root primordia in the absence of water stress [43].

According to [24], the factors determining the rooting and sprouting of leafy softwood cuttings are very different, depending on photosynthates produced in the propagation bed, while hardwood cuttings depend on the hydrolysis and availability of carbohydrates stored within the stem tissues. Vegetative propagation of G. kola by stem cuttings can be highly associated with presence of leaves which produce significant level of endogenous auxins and provide more stimuli on the base of cutting to promote rooting without using exogenous rooting hormone application.

The best mean root number per cutting observed on hardwood cutting with 28.25 and 56.5 cm² leaf area suggested that these leaf areas can promote root number. The best mean primary root length observed to greenwood cuttings with 113 cm² leaf area suggested that this leaf area produce sufficient carbohydrates, hormones and co-factors for root elongation. The lowest primary root length of leafless cutting and cutting with 28.25 cm² leaf area shows the importance of the leaf on G. kola cutting propagation. During rooting process, shooting and leafing contribute to enhance rooting. In case of G. kola, shoot developed first and produce endogenous rooting hormones which come down on the base of cuttings. The importance of leaves and new shoots in cuttings relies on the fact that these organs produce plant hormones, such as indoleacetic acid, and rooting cofactors, both essential for adventitious root formation which moves to the base of cutting to promote root initiation [44]. Rooting process starts by the callus formation on the base of the cutting.

The well survival percentage of regenerated plants and its growth in the forest may be due to its vigour. The regenerated plants presented for those cuttings two primary roots which promote the consolidation of the plant. The rooting quality and the high number of secondary roots promote well nutrition and growth activation. On the other end, the well survival rate can be explained by the best adaptation of regenerated plants of the climatic conditions of the forest. This result is essential for the species biodiversity conservation through its introduction in the forest. The cultivation of the species should be possible by using regenerated plants from cuttings.

This study indicates that regeneration of G. kola by seedling from stem cuttings is possible and this was not requiring necessary using exogenous hormones and sophisticated equipment. Non-mist poly-propagator provides well conditions for stem cuttings propagation success. Greatest success was achieved using hardwood cuttings with 113 cm² leaf area. Further work should seek the optimal conditions for large-scale production of selected G. kola cultivars for cultivation by local farmers. Such conditions are appropriate for use in remote locations in tropical countries, even without purchased rooting hormones. The best survival rate and growth of regenerated plants in forest represent an important step for the species cultivation and domestication through agroforestry systems.

This study was funded by the International Foundation for Science (IFS), Project IFS Garcinia (D/5274-2).

The authors declare no conflicts of interest regarding the publication of this paper.

Dao, J.P., Kouakou, K.L., Kouakou, C., Cherif, M., Ouedraogo, M.H., Koffi, K.K. and Bi, I.A.Z. (2020) Effect of Leafy and Leafless Greenwood, Softwood and Hardwood Cuttings Success of Garcinia kola (Heckel). Agricultural Sciences, 11, 897-911. https://doi.org/10.4236/as.2020.1110058