OJMMOpen Journal of Medical Microbiology2165-3372Scientific Research Publishing10.4236/ojmm.2020.103014OJMM-102933ArticlesMedicine&Healthcare Microflora Formation in Newborns Depending on the Mother Microbiological Profile and Locality Altitude BibigulOrunbaeva1KursantbekAttokurov2GulmiraAttokurova3ZhypargulAbdullaeva4*FeruzaAytieva5DilfuzaTuychieva6Anatomy, Histology and Normal Physiology Department, International Medical Faculty, Osh State University, Jolon Mamytov Campus, Osh, KyrgyzstanDepartment of Natural Sciences and Mathematics, International Medical Faculty, Osh State University, Osh, KyrgyzstanDepartment of General Medical Disciplines, College of Medicine, Osh State University, Osh, KyrgyzstanDepartment of Internal Diseases, Medical Faculty, Osh State University, Osh, KyrgyzstanDepartment of Zoology and Biochemistry, Andijan State University, Andijan, UzbekistanDepartment of Natural and Fundamental Sciences, Osh State University, Medical College, Osh, Kyrgyzstan30062020100316216914, August 202015, September 2020 18, September 2020© Copyright 2014 by authors and Scientific Research Publishing Inc. 2014This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/

Aims: T his work is aimed to investigate microflora formation, quantitative and qualitative features of various bacteria colonization in the women in labor, and newborns depending on the microbiological profile and locality altitude. Rationale: Physiological and social adaptation of newborns in extreme climate of mountains, in particular the microflora formation in unusual conditions studied. Findings: In the study of nasal microflora, the C. albicans species in case of women in labor were 77.7%, while in the newborns C. albicans species were 2 times less. In lowlands, the S. aureus type was prevailed, while in case of midlands prevailed bacterium was S. epidermidis. In the newborns of highlands, E. coli was prevailed, as in the examined lowlands , it found as 75%. In the women in labor , C. albicans were detected as 56.6%, C. krusei was 36.6%, S. aureus was 23.3%, S. epidermidis was 60% and E. coli was 30%. In newborns, representatives of candida fungi found: C. albicans was 3.3%, C. krusei was 26.6%, S. aureus was 43.3%, S. epidermidis was 53.6% and E. coli was 36.6%. Conclusions: In this work, we determined bacterial microflora colonization in throat, nasal, vaginal, urinoculture and skin swab of women in labor and newborns in lowland, midland and highland conditions, respectively.

Microflora Woman in Labor Newborns Microbiological Profile Bacteria1. Introduction

Microflora formation in newborns has potential to influence and impact the physical and neurocognitive development; numerous species and strains of bacteria exist in the neonatal microbiota [1] [2]. According to the UN Commission on Sustainable Development, the well-being of half of humanity in the XXI century related to the quality of development and development of mountainous territories [3]. As it is known, the species composition of human microflora is constantly changing, and its stability is considered as a relative due to alterations caused by bacterial infections [4], pharmacodynamic and pharmacokinetic of selected antibiotics [5], lifestyle and the dietary alterations inducing temporary microbial shifts within one day [6] [7]. Newborns early life immune system is not stable. It is rapidly adapting to environmental conditions and interventions aimed to protect organism against various pathogens and disorders [8] as represented in Figure 1. Conditions for initial microbiota colonization in infants are resulting in a symbiotic relationship between the colonizing bacteria and intestinal epithelial with lymphoid tissues [9] as represented in Table 1.

2. Research Methods

Studies conducted in various heights in the southern region of Kyrgyzstan, under examination of 133 women in labor aged from 17 to 43 years old, and 133 newborns. Examined people divided into three groups according to the locality altitude in which they are living:

1) Residents of the lowlands, Kurshab at 1012 m above the sea level;

2) The inhabitants of the middle mountains, Zhalpak-Tash at 2000 - 2500 m above the sea level;

3) The inhabitants of the highlands Sary-Tash, at 3325 m above the sea level.

Phases of intestinal colonization of the infant intestine
Phase oneIntrauterine period
The fetus becomes exposed to maternal microbiota through transplacenta passage into amniotic fluid
Phase two1st week of life
The newborn ingests maternal vaginal/colonic microbiota with passage through the birth canal (full term, vaginal delivery)
Phase threeTwo weeks to four months
Introduction of oral liquid feedings
Phase fourFour months to one year
Period of weaning to solid foods
Phase fiveOne to three years
Infant receives table food and intestinal microbiome resembles that of adult intestine (diversity of bacteria with greater than 1000 species)

Healthy women in labor and newborns considered according to the inclusion criteria. Studies conducted all year round. Our purpose was to determine quantitative and qualitative values of various bacterial growth and colonization in the healthy women in labor and newborns microscopically. Variables were excluded in this research. Bacteriological studies carried out in accordance with the Order of the Ministry of Health of the Kyrgyz Republic No. 4 from January 11.2010.11, “Methodological instructions for bacteriological methods of laboratory research of clinical material”. Work carried out at the Laboratory of Microbiology, Immunology and Virology of the Institute of Medical Problems of the Southern Branch of the National Academy of Sciences of the Kyrgyz Republic.

Microbiological methods such as isolation of bacteria, method of culture and identification of microbiota applied in this study. Types of samples: throat and skin swabs, nasal mucus, urine culture tested for microflora content.

3. Results and Discussions

In this study the throat microflora of women in labor and newborns in lowland conditions examined, during which the C. albicans bacteria [10] [11] detected in 19.4% mothers and in 6.9% of newborns, respectively (Figure 2). In the midland and highland conditions, this microflora was not found in the pharynx. In pharynx microflora of women in labor and newborns, C. krusei [12] bacteria observed only in lowland conditions. In the midland and highland, C. albicans bacteria not detected. S. aureus [13] found in all three studied groups: in lowlands, it was 8.4% in women in labor and 5.5% in newborns. In midland conditions, the S. aureus bacteria found as 23.3% in women in labor, and in newborns, it was 20%, which is 17.3% and 26.6% higher than in the lowland group. In highlands of women in labor, S. aureus noted as 35.4% of cases and in 25.8% in newborns, which is almost 3 times more in comparison with the lowland group.

In the study of nasal microflora (Figure 3), we found the C. albicans species in case of women in labor were 77.7%, while in newborns C. albicans species were 2 times less. In women of the midlands, this indicator reduced and amounted to 53%, while in newborns it found only in 13% of cases, which was amounted to 34.6% in relation to the lowlandgroup. In women of the alpine group, this indicator was 45%, while in newborns the C. albicans bacteria not found. C. krusei bacteria found in the women in labor in low and middle mountain conditions noted as almost 40% of cases, in newborns two times less. Figure 3 is showing microflora of nose in highland conditions, where C. albicans not detected in newborns. S. aureus bacteria in all groups were three times less in newborns comparing to the women in labor.

S. epidermidis bacteria dominated in the urine culture of women in labor at highlands, midlands and lowlands, as well as in newborns at lowland and highlands, at highest percentage (Figure 4). In newborns, urine culture at midlands contained dominated S. aureus bacteria. In the lowland conditions, in 72 women in labor C. albicans presented as 11%, S. aureus was 15%, and S. epidermidis was 25%. E. coli found 22%, P. rettrgeri was 22%, P. mirabilis was 31%, and P. vulgaris was 15%. In newborns urine culture C. albicans was detected 13%, S. aureuswas 12%, and S. epidermidis was 20%, microbe species of the P. rettgeri, P. mirabilis, P. vulgaris and Klebsiella observed too. In the midland conditions, 30 women in labor and 30 newborns examined where from the pathogenic microflora the following bacteria species detected:

S. aureus was 40%, S. epidermidis was 50%, C. albicans was 9% - 30%, and E. coli bacteria were 15%. In highlands, 31 women in labor and newborns examined where for women in labor the highest percentage of microflora dominated by S. Epidermidis species.

Skin swab microflora of women in labor and newborns analyzed for presence of bacteria. In lowlands, the S. aureus type was prevailed, while in case of midlands prevailed bacterium was S. epidermidis (Figure 5). In the newborns of highlands, E. coli was prevailed, as in the examined lowlands it found as 75%. All microflora representatives in the woman in labor and in newborns were 79%, in the woman in labor of midlands were 66%, and in newborns were 58%. In the highlands of the examined women in labor microflora bacteria were 31.1%, while in newborns were 22%.

In the lowland conditions, skin swab microflora examination was performed for 72 women in labor, where C. albicans was 22.2%, C. krusei was 18%, S. aureus was 22%, S. epidermidis was 12.5%, E. coli was 19.4%. In newborns, C. albicans found as 18.0%, C. krusei was 16.6%, S. aureus was 19.4%, S. epidermidis was 9.7%, and E. coli was 11% - 15.2%. As can be seen from the table, a high percentage of S. aureus observed for women in labor and newborns.

In the midland conditions, 30 women in labor and newborns examined. In the women in labor C. albicans were detected as 56.6%, C. krusei was 36.6%, S. aureus was 23.3%, S. epidermidis was 60% and E. coli was 30%. In newborns, representatives of candida fungi found: C. albicans was 3.3%, and C. krusei was 26.6%, S. aureus was 43.3%, S. epidermidis was 53.6% and E. coli was 36.6%. In case of highlands 31 inhabitants including woman in labor and newborns examined, where microflora of woman in labor showed C. albicans as 51%, C. krusei was 54%, S. aureus was 67.7%, S. epidermidis was 93.5%, and E. coli was 51%. In the newborns C. albicans was 38.7%, C. krusei was 29%, S. aureus was 35.4%, S. epidermidis was 61%, and E. coli was 58%.

4. Conclusion

Throat examination of woman in labor and newborns in midlands and highlands showed no candida detected. In the low mountain conditions 1012 meters above sea level, a higher percentage of candida observed for women in labor, while in newborns epidermal staphylococcus dominated. Nose microflora in lowlands showed a high percentage of C. albicans and S. aureus bacteria in both mothers and newborns. In the midlands, the large number of S. aureus observed for women in labor, and in newborns, S. epidermidis found, while in the newborns of highlands, this yeast not detected. In newborns, urine culture at midlands contained dominated S. aureus bacteria. Skin swab microflora of women in labor and newborns analyzed for presence of bacteria. In lowlands, the S. aureus type was prevailed, while in case of midlands prevailed bacterium was S. epidermidis.

Conflicts of Interest

The authors declare no conflicts of interest regarding the publication of this paper.

Cite this paper

Orunbaeva, B., Attokurov, K., Attokurova, G., Abdullaeva, Z., Aytieva, F. and Tuychieva, D. (2020) Microflora Formation in Newborns Depending on the Mother Microbiological Profile and Locality Altitude. Open Journal of Medical Microbiology, 10, 162-169. https://doi.org/10.4236/ojmm.2020.103014

ReferencesYang, I., Corwin, E.J., Brennan, P.A., Jordan, S., Murphy, J.R. and Dunlop, A. (2016) The Infant Microbiome: Implications for Infant Health and Neurocognitive Development. Nursing Research, 65, 76-88. https://doi.org/10.1097/NNR.0000000000000133Fanaro, S., Chierici, R., Guerrini, P. and Vigi, V. (2003) Intestinal Microflora in Early Infancy: Composition and Development. Acta Pædiatrica, 92, 48-55. https://doi.org/10.1111/j.1651-2227.2003.tb00646.xMensah, J. and Casadevall, S.R. (2019) Sustainable Development: Meaning, History, Principles, Pillars, and Implications for Human Action: Literature Review. Cogent Social Sciences, 5, 1. https://doi.org/10.1080/23311886.2019.1653531Clemente, J.C., Ursell, L.K., Parfrey, L.W. and Knight, R. (2012) The Impact of the Gut Microbiota on Human Health: An Integrative View. Cell, 148, 1258-1270. https://doi.org/10.1016/j.cell.2012.01.035Kim, S., Covington, A. and Pamer, E.G. (2017) The Intestinal Microbiota: Antibiotics, Colonization Resistance, and Enteric Pathogens. Immunological Reviews, 279, 90-105. https://doi.org/10.1111/imr.12563Rodríguez, J.M., Murphy, K., Stanton, C., Ross, R.P., Kober, O.I., Juge, N., Avershina, E., Rudi, K., Narbad, A., Jenmalm, M.C., Marchesi, J.R. and Collado, M.C. (2015) The Composition of the Gut Microbiota throughout Life, with an Emphasis on Early Life. Microbial Ecology in Health and Disease, 26, 26050. https://doi.org/10.3402/mehd.v26.26050Singh, R.K., Chang, H.W., Yan, D., Lee, K.M., Ucmak, D., Wong, K., Abrouk, M., Farahnik, B., Nakamura, M., Zhu, T.H., Bhutani, T. and Liao, W. (2017) Influence of Diet on the Gut Microbiome and Implications for Human Health. Journal of Translational Medicine, 15, 73. https://doi.org/10.1186/s12967-017-1175-yKollmann, T.R., Kampmann, B., Mazmanian, S.K., Marchant, A. and Levy, O. (2017) Protecting the Newborn and Young Infant from Infectious Diseases: Lessons from Immune Ontogeny. Immunity, 46, 350-363. https://doi.org/10.1016/j.immuni.2017.03.009Walker, W.A. (2017) The Importance of Appropriate Initial Bacterial Colonization of the Intestine in Newborn, Child, and Adult Health. Pediatric Research, 82, 387-395. https://doi.org/10.1038/pr.2017.111Kim, J. and Sudbery, P. (2011) Candida albicans, a Major Human Fungal Pathogen. Journal of Microbiology, 49, 171. https://doi.org/10.1007/s12275-011-1064-7Dunn, A.B., Jordan, S., Baker, B.J. and Carlson, N.S. (2017) The Maternal Infant Microbiome: Considerations for Labor and Birth. MCN. The American Journal of Maternal Child Nursing, 42, 318-325. https://doi.org/10.1097/NMC.0000000000000373Fleischmann, J., Broeckling, C.D. and Lyons, S. (2017) Candida krusei form Mycelia along Agar Surfaces towards Each Other and Other Candida Species. BMC Microbiology, 17, 60. https://doi.org/10.1186/s12866-017-0972-zPerez-Muñoz, M.E., Arrieta, M.C., Ramer-Tait, A.E. and Walter, J. (2017) A Critical Assessment of the “Sterile Womb” and “in Utero Colonization” Hypotheses: Implications for Research on the Pioneer Infant Microbiome. Microbiome, 5, 48. https://doi.org/10.1186/s40168-017-0268-4