The nanotechnology is more beneficial in many applications specifically in the medical field as nanomedicine in therapy and diagnosis of cancer [1]. The cancer is a state of abnormal cell growth as the malignant tumor or malignant neoplasm, with a potential to invade other cells or organs in the body [2]. The most common types of cancers are stomach, lung, breast, colon-rectum, prostate, cervix uteri, mouth, pharynx, liver and esophagus. Its intricacy relies on being a class or group of combined diseases which makes it extremely challenging to find a single cure and target a specific tissue [3] [4]. The current cancer therapy approaches are based on surgery, radiotherapy and chemotherapy where the latter is the one that shows the greater efficacy for treatment, expressly in advanced stages [5]. Many materials have been used in nanotechnology for cancer diagnosis and therapy [6]. The gold nanoparticles (GNPs) are the most compatible nanomaterial for the preparation of engineered nanoplatforms in smart sensing devices. The surface plasmon resonance of GNP makes them the most suitable engineered nanomaterial for bioimaging, biomedical therapeutics and biodiagnostic tools [7]. They are named as gold colloids, and have attracted increasing attention due to their unique properties in multi-disciplinary research fields [8] [9].

The gold nanoparticles are sensitively strong with the light by their environment, size and physical dimensions. The oscillating electric fields of a light ray propagating near a colloidal nanoparticle interact with the free electrons causing a concerted oscillation of electron charge that is in resonance with the frequency of visible light. These resonant oscillations are known as surface plasmon resonance. The small monodispersed gold nanoparticles are about (~30 nm). The surface plasmon resonance phenomenon is producing a light absorption in blue-green portion of the spectrum at frequency of about (~450 nm). While, the red light at (~700 nm) is reflected, yielding a rich red color. As particle size increases, the wavelength of surface plasmon resonance related absorption shifts to longer, redder wavelengths. Red light is then absorbed, and blue light is reflected, yielding solutions with a pale blue or purple color as shown in Figure 1. As the particle size increases toward the bulk limit, surface plasmon resonance wavelengths move to the IR spectrum and most visible wavelengths are reflected, giving the nanoparticles clear or translucent color. The surface plasmon resonance can be tuned by varying the size or shape of the nanoparticles, leading to particles with tailored optical properties for different applications.

The nanotechnology and nanomaterials have attracted large attention in diverse research areas. In recent years, the gold nanoparticles have been used in different fields owing to their unique optical and electrical properties [10] [11]. The gold nanoparticles (GNPs) are defined as stable colloidal solutions of cluster gold atoms with sizes ranging from one to a hundred nanometers. The nanospherical gold possesses different physical and chemical characteristics rather than the bulk one [12] [13].

In the medical field, the advantages of gold nanostructure in size depend on surface Plasmon resonance (SPR), easy-surface functionalization and strong X-ray absorption make GNPs particularly useful in a radiation therapy, photo thermal therapy and biosensors [11]. The spectrum of surface plasmon absorption

does not depend on the size but also on the shape of gold nanostructures. When the shape of the gold nanostructure changes from nanospheres to nanorods, the surface Plasmon absorption spectrum also changes [14]. In the case of gold nanosphere shape, the spectrum has a strong absorption band around (520 nm) at the resonant frequency with the coherent electron motion in the conduction band of the gold nanoparticles [15]. The preparation of uniform gold nanoparticles (AuNPs) is one of the most common procedure in the chemical reduction method (Turkevich method) by the presence of Au³⁺ aqueous solution due to citrate ions near the boiling point (~100˚C) to produce gold nanoparticles in the form of a stable solution [16].

The colloidal nano-gold was prepared by the reduction method called Turkevich method [17]. 50 ml beakers were cleaned gently with double distilled water, then different molarities of gold chloride tetra hydride (HAuCl₄∙4H₂O) solutions were added to the beakers. These molarities were (0.1, 0.15, 0.2, 0.25 and 0.3) respectively. These solutions were heated on a hot plate and magnetic stirrer combination until it reached to 98˚C temperature. At this temperature, 1 ml of 17 mM trisodium citrate solution was added quickly while observing the colors of the final solutions which were a ruby red colored of nano-gold particles that appeared. The reduction time was 10 minutes for all the experiments and the final solution of the gold nanoparticles was a ruby red colored solution. Two techniques were used to emphasize the presence of gold nanostructure, such as UV-visible absorption spectrum, and transmission electron microscope (TEM). Then using 4 ml quartz cavity filled by a colloidal nanogold solution for UV-visible. A drop of nanogold solution was evaporated onto a carbon-coated copper grid and allowed to dry for the transmission electron microscope analysis.

After the successful preparation of nano-gold solutions with different concentrations, the UV-Visible absorption spectrum is the tool to show the surface Plasmon resonance. The absorption spectrum exhibited the presence of nanoparticle of a gold solution as shown in Figure 2. The difference in the spectrum appeared in the concentrations of the nano-gold solution in the range (0.1 - 0.3 mM) by step 0.5 mM. The peak position of the surface Plasmon resonance was in the range (524 - 520 nm), as mentioned in Figure 3, with different concentrations. This range is limited increasing with the previous one [16] [18]. It is clear the absorption intensity increased with increasing the gold salt molarity.

The output data appear in Table 1 that describes the relationship between the wavelengths of the surface Plasmon resonance peak with the gold nanoparticles size. It describes the dependence on the ratio of the absorption intensity at surface Plasmon resonance peak to the absorption intensity at the lower peak closed to the surface Plasmon peak. The calculated theoretical diameter of the gold nanoparticle is compared with the experimental data gotten from TEM as shown in

Figure 4 and Figure 5, and the standard one by Sigma-Aldrich results. The transmission electron microscopy (TEM) is a good technique to observe the size and the shape of the gold nanostructures, as shown in Figure 4 and Figure 5. The general view showed the presence of nanoparticles of gold. It appeared as a spherical shape with average particle size in the range (10 - 11 nm), they had high monodispersity. The TEM analysis is matched with Table 1, for two molarities 0.15 and 0.25 mM. They are approaching the standard one concluded by Sigma-Aldrich [18]. On the other hand, the presence of nanoparticles through the accumulation of these nanospheres producing nanobelt, as mentioned in Figure 5. The appearance ratio for the particle size is about (10 - 11 nm) with the ratio 41%, and the ratio 15% for the particle size (20.74 nm) with the ratio of 15%. This accumulation is more useful in the treatment of cancer cells in the human body, so the production of nanobelt is more efficient than the nanoparticle.

The electrical conductivity of colloidal containing gold nanostructures was studied as a function of different molarity and room temperature, as shown in Figure 6. It is normally the conductivity of colloidal increase as the molarity increased for nanostructure. This is might be attributed to the presence of a high concentration of nanoparticles. This is the reason for the accumulation Au-ions as the molarity increased producing high electrical conductivity at room temperature. The change in the conductivity is tentatively attributed to the ion dissociation from the nanoparticle surface [19]. On the other hand, the total dissolved solids (TDS) is related to the concentration of dissolved solid particles in the solution. The TDS is a combination of all ion particles that are smaller than 2 microns. This includes all of the disassociated electrolytes that make up salinity concentrations, as well as other compounds such as dissolved organic matter. The behavior of TDS as a function of molarity is shown in Figure 7. As the molarity of the gold nanoparticles increased, TDS also increased.

There is a matching between TDS and the conductivity that means a high dissolving of gold ions in the solution tends to make high conductivity. This result is confirmed in Figure 6. While TDS measurements are derived from conductivity, and it often sets a TDS maximum instead of a conductivity limit for water quality.

Whereas, measuring of the pH-value is considered the main role in understanding the increase of conductivity and TDS-value as a function of molarity, as mentioned in Figure 8. It describes the power of hydrogen (pH) that is produced in synthesized gold nanoparticles solutions at different molarities. As the gold salt molarity increased, pH decreased and this attributed to the acidity of the nanogold solution. The varying range of pH-value is (6.2 - 5.4) with the bowing value of about (4.6) appeared at molarity (0.2 mM). The general view of the solution including gold nanoparticle is in the acid shape, which is the reason for increasing the conductivity.

The preparation of gold nanoparticles makes their accumulation in the solution to produce nanobelt as confirmed by the TEM analysis. The surface plasmon resonance phenomena are the controlling system in the synthesis of gold nanoparticles solution by increasing the concentrations of the gold salt through the molarity. It was noticed that increasing the gold salt molarity leads to increasing the ionic conductivity. The total dissolved solids (TDS) and the conductivity of the nanogold solution depend on the molarity. The pH-value was varied in the acidity range that is the reason for increasing the conductivity. These results are more beneficial in the medical application during the treatment of cancer cells in the human body because the high conductivity makes it easier to accumulate the gold nanoparticle making a wall like or creating nanobelt.

The authors declare no conflicts of interest regarding the publication of this paper.

Badi, S.J, Al-Shakarchi, E.K. and Ahmed, S.J. (2020) The Effect of Gold Salt Concentration in the Production of Gold Nanospheres. Journal of Applied Mathematics and Physics, 8, 1487-1495. https://doi.org/10.4236/jamp.2020.88114