1. Introduction

Chinese Medicine

2151-1918

Scientific Research Publishing

10.4236/cm.2020.111002

CM-100706

Articles

Medicine&Healthcare

Exploration on Shufeng Jiedu Capsule for Treatment of COVID-19 Based on Network Pharmacology and Molecular Docking

Yanwu

Zhao

¹^*Junhui

¹Jiang

Song

¹Xinhong

Zhao

¹Yanjing

Shi

¹Yanping

Jiang

Department of Pharmacy, The Affiliated Hospital of Chengde Medical College, Chengde, China

04062020

11019183, February 202028, March 2020 31, March 2020

2014

This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/

The paper is proposed to explore the potential effects of Shufeng Jiedu Capsule against COVID-19. The ingredients and targets of Shufeng Jiedu Capsule were collected by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), and the gene names of potential targets were extracted by UniProtKB. Then we did protein-protein interaction networks functional enrichment analysis by the STRING platform, reconstructed drug-target pathways and networks to predict the likely protein targets of the capsule against COVID-19 with software Cytoscape 3.6.1, and carried out GO enrichment analysis and KEGG analysis with R 5.3.2 software. At last we validated our predictions on molecular docking. The results suggested that Shufeng Jiedu Capsule contained 155 ingredients and 237 targets, including 26 main active ingredients and 45 key targets. There were 2334 biological processes (BP), 103 cell composition (CC) and 198 molecular functions (MF) in GO Enrichment Analysis, and 177 pathways in the KEGG analysis. The molecular docking analysis showed that binding energy for 26 main active ingredients ranged from -32.21 to -25.94 kJ·mol^-1, and the main targets bind to SARS-CoV-2 3CL hydrolase by acting on CASP9, PRKCA, RELA and others. Our study suggested that Shufeng Jiedu Capsule has potential therapeutic effects on COVID-19.

Shufeng Jiedu Capsule Network Pharmacology Molecular Docking COVID-19

1. Introduction

Coronavirus disease 2019 (COVID-19) is caused by a novel coronavirus and is highly infectious. It was named by the World Health Organization on January 12, 2020, and its pathogen was named SARS-CoV-2 [1]. From the traditional Chinese medicine angle, COVID-19 is a blight [2] [3]. Chinese medicine therapy is recommended from the third edition of “Novel Coronavirus Pneumonia Diagnosis and Treatment Scheme” issued by National Health Commission of the People’s Republic of Chian and National Administration of Traditional Chinese Medicine, and achieved good results in treatment [4] [5] [6] [7]. Shufeng Jiedu Capsule was recommended from the sixth edition of “Novel Coronavirus Pneumonia Diagnosis and Treatment Scheme”; the formula was from “Qudu San”, which is made from Polygoni Cuspidati Rhizoma Et Radix (Huzhang), Forsythiae Fructus (Lianqiao), Isatidis Radix (Banlangen), Radix Bupleuri (Chaihu), Herba Patriniae (Baijiangcao), Verbenae Herb (Mabiancao), Phragmitis Rhizoma (Lugen), and licorice (Gancao). The capsule has the functions of expelling wind, clearing heat and detoxification. Clinical studies showed that the capsule had antibacterial, antiviral and immunomodulatory effects, and had a good effect in the treatment of acute upper respiratory tract infection and community-acquired pneumonia [8] [9] [10] [11]. In this study, we selected relevant targets and pathological mechanisms of Shufeng Jiedu Capsule for the treatment of COVID-19 by network pharmacology and molecular docking [12], and then provided the basis for further study.

2. Materials and Methods2.1. The Main Active Components and Corresponding Targets of Shufeng Jiedu Capsule

Searched names of 8 the traditional Chinese medicine (TCM) in Shufeng Jiedu Capsule as Polygoni Cuspidati Rhizoma Et Radix (Huzhang), Forsythiae Fructus (Lianqiao), Isatidis Radix (Banlangen), Radix Bupleuri (Chaihu), Herba Patriniae (Baijiangcao), Verbenae Herb (Mabiancao), Phragmitis Rhizoma (Lugen), licorice (Gancao), selected the active components and corresponding targets with filter conditions as oral bioavailability (OB) ≥ 30, drug-like (DL) ≥ 0.18.

2.2. Constructed Drug-Compound-Target Network

The gene symbols of the active components of Shufeng Jiedu Capsules were extracted from UniProtKB (http://www.uniprot.org), and the target was limited to Homo sapiens. Then construct PPI network with filter conditions as median confidence > 0.4. Then construct drug-compound-target network by software Cytoscape 3.6.1, then select potential active components of Shufeng Jiedu Capsule against COVID-19 according to the node degree method [13].

2.3. Gene Ontology Analysis and Pathway Analysis

The targets of active components of Shufeng Jiedu Capsule were analyzed by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. GO analysis included Biological Process (BP), Molecular Function (MF), Cellular Component (CC). Two analyses were worked on software R 5.3.2 with package of “org.Hs.eg.db”, “clusterProfiler”, “enrichplot” and “ggplot2” [14], adjust P < 0.05.

2.4. Molecular Docking

Download the active ingredients of Shufeng Jiedu Capsule from TCMSP and saved them in mol2 format, then saved them in PDBQT format through software Auto Dock. The 3D structure (PDB ID: 6LU7) of SARS-CoV-2 3CL hydrolase and angiotensin converting enzyme II (ACE2) download from RCSB database (http://www.rcsb.org/), then saved in PDB format, removed water and other small molecules, added hydrogenation through software Auto Dock, then saved in PDBQT format. Made molecular docking by software Vina (http://vina.scripps.edu/index.html), then selected the minimum binding energy of drug and protein as the optimal site, viewed and saved images by software Discovery Studio 2016.

3. Results3.1. The Main Active Components and Corresponding Targets of Shufeng Jiedu Capsule

Selected 207 components of Shufeng Jiedu Capsule through TCMSP platform, included 10 of Polygoni Cuspidati Rhizoma Et Radix (Huzhang), 23 of Forsythiae Fructus (Lianqiao), 39 of Isatidis Radix (Banlangen), 17 of Radix Bupleuri (Chaihu), 13 of Herba Patriniae (Baijiangcao), 12 of Verbenae Herb (Mabiancao), 1 of Phragmitis Rhizoma (Lugen), 92 of Phragmitis Rhizoma (Gancao).

3.2. Constructed Drug-Compound-Target Network

Drug-compound-target network of Shufeng Jiedu Capsule includes 400 nodes, 7756 edges, 8 TCM, 155 active compounds, 236 targets. As shown in Figure 1, green—TCM, yellow—compounds, red—targets. Our study also selected key nods according to degree of topological analysis, including 3 TCM, 45 targets, 26 compounds (shown in Table 1).

Table 1 Main active components of Shufeng Jiedu Capsule

	Mol ID	Components	MW	OB (%)	DL
Huzhang	MOL013287	Physovenine	262.34	106.21	0.19
	MOL000358	beta-sitosterol	414.79	36.91	0.75
	MOL000006	luteolin	286.25	36.16	0.25
	MOL000098	quercetin	302.25	46.43	0.28
Lianqiao	MOL000173	wogonin	284.28	30.68	0.23
	MOL000358	beta-sitosterol	414.79	36.91	0.75
	MOL000422	kaempferol	286.25	41.88	0.24
	MOL000006	luteolin	286.25	36.16	0.25
	MOL000791	bicuculline	367.38	69.67	0.88
	MOL000098	quercetin	302.25	46.43	0.28
Banlangen	MOL001689	acacetin	284.28	34.97	0.24
	MOL000358	beta-sitosterol	414.79	36.91	0.75
	MOL000449	Stigmasterol	412.77	43.83	0.76
Baijiangcao	MOL001689	acacetin	284.28	34.97	0.24
	MOL000358	beta-sitosterol	414.79	36.91	0.75
	MOL000422	kaempferol	286.25	41.88	0.24
	MOL000449	Stigmasterol	412.77	43.83	0.76
	MOL000006	luteolin	286.25	36.16	0.25
	MOL000098	quercetin	302.25	46.43	0.28
Chaihu	MOL000449	Stigmasterol	412.77	43.83	0.76
	MOL000354	isorhamnetin	316.28	49.6	0.31
	MOL000422	kaempferol	286.25	41.88	0.24
	MOL000098	quercetin	302.25	46.43	0.28
Mabiancao	MOL000358	beta-sitosterol	414.79	36.91	0.75
	MOL000422	kaempferol	286.25	41.88	0.24
	MOL000449	Stigmasterol	412.77	43.83	0.76
	MOL000006	luteolin	286.25	36.16	0.25
	MOL002773	beta-carotene	536.96	37.18	0.58
	MOL000098	quercetin	302.25	46.43	0.28
Lugen	MOL000449	Stigmasterol	412.77	43.83	0.76
Gancao	MOL002565	Medicarpin	270.3	49.22	0.34
	MOL000354	isorhamnetin	316.28	49.6	0.31
	MOL003896	7-Methoxy-2-methyl isoflavone	266.31	42.56	0.2
	MOL000392	formononetin	268.28	69.67	0.21
	MOL000422	kaempferol	286.25	41.88	0.24

MOL004328	naringenin	272.27	59.29	0.21
MOL004835	Glypallichalcone	284.33	61.6	0.19
MOL004891	shinpterocarpin	322.38	80.3	0.73
MOL004908	Glabridin	324.4	53.25	0.47
MOL004957	HMO	268.28	38.37	0.21
MOL004959	1-Methoxyphaseollidin	354.43	69.98	0.64
MOL004966	3'-Hydroxy-4'-O-Methylglabridin	354.43	43.71	0.57
MOL000497	licochalcone a	338.43	40.79	0.29
MOL004974	3'-Methoxyglabridin	354.43	46.16	0.57
MOL004978	2-[(3R)-8,8-dimethyl-3,4-dihydro-2H-pyrano[6,5-f]chromen-3-yl]-5-methoxyphenol	338.43	36.21	0.52
MOL000500	Vestitol	272.32	74.66	0.21
MOL005003	Licoagrocarpin	338.43	58.81	0.58
MOL000098	quercetin	302.25	46.43	0.28

3.3. Gene Ontology Analysis and Pathway Analysis

We got 2363 GO items through Gene ontology analysis, including 2334 BP items, 103 CC items, 198 MF items. We also got 177 pathways through KEGG analysis, including Human cytomegalovirus infection, PI3K-Akt signaling pathway and Hepatitis B, etc. The results are shown in Figure 2.

3.4. Molecular Docking

Molecular docking with SARS-CoV-2 3CL hydrolase was carried out between the 26 main active components of Shufeng Jiedu Capsule selected above. The lower the binding energy, the greater the possibility of interaction between the active compound and the hydrolase. The binding energy of 26 compounds is from −32.21 to −20.94 kJ·mol⁻¹ (shown in Table 2). Bicuculline (MOL000791) had the greatest effect with hydrolase (−32.21 kJ·mol⁻¹), which is the active compound of Forsythiae Fructus (Lianqiao), the second was MOL004978 (−30.54 kJ·mol⁻¹), the third was MOL004966 (−30.12 kJ·mol⁻¹), the results are shown in Figure 3.

4. Discussion

Currently, there is no specific drug treatment for COVID-19, and existing chemical drugs can only inhibit some clinical symptoms within a limited range, but within higher cure rate and lower mortality rate of confirmed patients with TCM treatment [15]. The sixth edition of “Novel Coronavirus Pneumonia Diagnosis and Treatment Scheme” advised different TCM treatments according to TCM dialectics in treatment in patients in different pathological stages, and Shufeng Jiedu Capsule was recommended for prevention and treatment during medical observation stage.

Table 2 The binding energy of 26 compounds and SARS-CoV-2 3CL hydrolase

MOL ID	Compound	Shufeng Jiedu Capsule								binding energy (kJ·mol⁻¹)
MOL ID	Compound	HZ	LQ	BLG	BJC	CH	MBC	LG	GC	binding energy (kJ·mol⁻¹)
MOL000098	quercetin	√	√	—	√	√	√	—	√	−25.52
MOL000422	kaempferol	—	√	—	√	√	√	—	√	−25.10
MOL000006	luteolin	√	√	—	√	—	√	—	—	−28.45
MOL000358	beta-sitosterol	√	√	√	√	—	√	—	—	−25.94
MOL000449	Stigmasterol	—	—	√	√	√	√	√	—	−26.35
MOL000354	isorhamnetin	—	—	—	—	√	—	—	√	−24.68
MOL000173	wogonin	—	√	—	—	—	—	—	—	−25.94
MOL001689	acacetin	—	—	√	√	—	—	—	—	−24.68
MOL003896	7-Methoxy-2-methyl isoflavone	—	—	—	—	—	—	—	√	−23.84
MOL004328	naringenin	—	—	—	—	—	—	—	√	−26.35
MOL000392	formononetin	—	—	—	—	—	—	—	√	−28.86
MOL013287	Physovenine	√	—	—	—	—	—	—	—	−25.10
MOL000497	Licochalcone A	—	—	—	—	—	—	—	√	−25.94
MOL004891	Shinpterocarpin	—	—	—	—	—	—	—	√	−28.45
MOL004978	2-[(3R)-8,8-dimethyl-3,4-dihydro-2H-pyrano[6,5-f] chromen-3-yl]-5-methoxyphenol	—	—	—	—	—	—	—	√	−30.54
MOL002565	Medicarpin	—	—	—	—	—	—	—	√	−25.94
MOL000500	Vestitol	—	—	—	—	—	—	—	√	−24.26
MOL005003	Licoagrocarpin	—	—	—	—	—	—	—	√	−27.61
MOL002773	beta-carotene	—	—	—	—	—	√	—	—	−28.45
MOL004835	Glypallichalcone	—	—	—	—	—	—	—	√	−22.17
MOL004974	3'-Methoxyglabridin	—	—	—	—	—	—	—	√	−29.70
MOL000791	bicuculline	—	√	—	—	—	—	—	—	−32.21
MOL004908	Glabridin	—	—	—	—	—	—	—	√	−28.45
MOL004957	HMO	—	—	—	—	—	—	—	√	−20.92
MOL004959	1-Methoxyphaseollidin	—	—	—	—	—	—	—	√	−24.26
MOL004966	3'-Hydroxy-4'-O-Methylglabridin	—	—	—	—	—	—	—	√	−30.12

Note: 1) HZ—Polygoni Cuspidati Rhizoma Et Radix, LQ—Forsythiae Fructus, Isatidis BLG—Radix, CH—Radix Bupleuri, BJCb—Herba Patriniae, MBC—Verbenae Herb, LG—Phragmitis Rhizoma, licorice—GC. 2) √—contain compound, — —free from compound.

In this study, through network pharmacology and molecular docking, we got 26 active compounds of Shufeng Jiedu Capsule, and binding energy of compounds and SARS-CoV-2 3CL hydrolase were from −32.21 to −20.94 kJ·mol⁻¹. Quercetin (−25.52 kJ·mol⁻¹) in our study is similar to the published research [13], whose binding energy with SARS-CoV-2 3CL hydrolase was −26.36 kJ·mol⁻¹. The results of molecular docking are shown that combination of Shufeng Jiedu Capsule and hydrolase includes hydrogen bond, pi-pi conjugate and van der Waals force. Moreover, the capsule contains many active compounds, then it can be predicted that Shufeng Jiedu Capsule has a high possibility of anti-COVID-19.

Our study selected the main compounds and targets of Shufeng Jiedu Capsule by network pharmacology, then analyzed the potential molecular mechanism on its binding energy with SARS-CoV-2 3CL hydrolase by molecular docking. The results showed that Shufeng Jiedu Capsule acts on hydrolase by multiple compounds, targets and pathways. The method in this study provides the basis in mechanism of action of Chinese herbal compound preparation. However, this study still has some limitations, such as insufficient understanding of the pathogenesis of COVID-19 and insufficient research on the key targets of the viral infection process. In this study, the pharmacodynamics, evaluation and clinical efficacy of COVID-19 were studied and discussed at a theoretical level. In the later stage, the pharmacodynamics, evaluation and clinical efficacy should be further studied to provide a basis for further development and utilization of COVID-19 therapeutic drugs.

Funding

This study was funded by the Study and Development Fund for Sciences and Technology in Chengde City (No. 201701A087).

Conflicts of Interest

The authors declare no conflicts of interest regarding the publication of this paper.

Cite this paper

Zhao, Y.W., Hu, J.H., Song, J., Zhao, X.H., Shi, Y.J. and Jiang, Y.P. (2020) Exploration on Shufeng Jiedu Capsule for Treatment of COVID-19 Based on Network Pharmacology and Molecular Docking. Chinese Medicine, 11, 9-18. https://doi.org/10.4236/cm.2020.111002

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