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F. Li, Y. Feng, Z. Wang, L. Yang, L. Zhuo and B. Tang, “Direct Electrochemistry of Horseradish Peroxidase Immobilized on the Layered Calcium Carbonate-Gold Nanoparticles Inorganic Hybrid Composite,” Biosensors and Bioelectronics, Vol. 25, No. 10, 2010, pp. 2244-2248.
http://dx.doi.org/10.1016/j.bios.2010.03.006
has been cited by the following article:
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TITLE:
Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Entrapped in Titania Sol-Gel Film on Screen-Printed Electrode
AUTHORS:
Reza E. Sabzi, Fereshteh Rasouli, Farshad Kheiri
KEYWORDS:
Screen-Printed Electrode; Ag Nanoparticle; Titania Sol-Gel; Biosensor
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.11,
November
11,
2013
ABSTRACT: We report the fabrication of disposable and flexible Screen-Printed Electrodes (SPEs). This new type of screen-printed electrochemical platform consists of Ag nanoparticles (AgNPs) and graphite composite. For this purpose, silver nanoparticles were first synthesized by a chemical reduction method. The morphology and structure of the AgNPs were analyzed using a Scanning Electron Microscope (SEM) and UV-Visible spectroscopy. Graphite was chosen as the working electrode material for the fabrication of a thick-film. The fabrication of a screen-printed hydrogen peroxide biosensor consisting of three electrodes on a polyethylene terephthalate (PET) substrate was performed with a spraying approach (working, counter and reference: enzyme electrode, graphite, pseudo reference: Ag/AgCl). This biosensor was fabricated by immobilizing the peroxidase enzyme (HRP) in a Titania sol-gel membrane which was obtained through a vapor deposition method. The biosensor had electrocatalytic activity in the reduction of H2O2 with linear dependence on H2O2 concentration in the range of 10-5 to 10-3 M; the detection limit was 4.5 × 10-6 M.