TITLE:
Assessment on early blight of potato in order to compare the two methods in vitro using pathogenic fungi Alternaria solani
AUTHORS:
Hamid Reza Mirkarimi, Ahmad Abasi-Moghadam, Javad Mozafari
KEYWORDS:
Early Blight; AUDPC; Resistance; Alternaria solani; Potato
JOURNAL NAME:
Natural Science,
Vol.5 No.11,
November
4,
2013
ABSTRACT: Potato (Solanum
tuberosum) early blight, caused by Alternaria solani is one of the
most destructive fungal foliar diseases. This research was done in order to
study methods comparison of evaluation by culture filtrate of A. solani in in vitro condition for selecting resistance
cultivars to early blight. Plantlets of potato viruse free were obtained from
the National plant gene bank of Iran, and were inoculated in vitro methods with a culture filtrate of A. solani. In in vitro selection
by droplet of culture filtrate method, leaflet received a 10 μl droplet of the A.
solani culture filtrate and in in vitro selection by direct using of
culture filtrate method, plantlets were placed in test tubes that include 5 μl A.
solani culture filtrate. The experimental design was factorial on basis of completely
randomized design (CRD) with two factors, three replications and six genotypes.
During droplet method assay, the A. solani symptoms appeared 1 - 2 days
until 6 days and during direct method they appeared 2 - 3 days until 6 days.
The AUDPC values were submitted to the analysis of varience (ANOVA) and AUDPC
means were compared by using Duncan test (α = 0.01%). In each method, significant
difference among potato cultivars was observed for disease to early blight (p in vitro methods experiment had the same result.