TITLE:
Biochemical variability between two Egyptian Stenodactylus species (Reptilia: Gekkonidae) inhabiting North Sinai
AUTHORS:
Mohamed A. M. Kadry, Sayed A. M. Amer
KEYWORDS:
Electrophoreses; Physiological Ecology; Geckos; Isoenzymes; Lipids; Proteins
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.11,
November
1,
2013
ABSTRACT: Polyacrylamide gel electrophoreses for malate dehydrogenase (Mdh) and beta-esterase (β-Est) isoenzymes were
conducted for biochemical differentiation between two Stenodactylus gekkonid
species inhabiting North Sinai of Egypt. Total lipids and proteins of liver and
muscle tissues in both species were also analyzed. A total of three Mdh isoforms
were recorded in the analysis, in which the activity of Mdh-2 and Mdh-3 seemed to
be higher in S. petrii than in S. sthenodactylus. This high
activity could be supported by the significant increase in the total lipids and
proteins in liver and muscle tissues of the species. It may thus be
reasonable to suppose that S. petrii is more active, energetic
and adaptable in the desert habitat than S. sthenodactylus. β-Est showed
six fractions in S. petrii and only one fraction in S. sthenodactylus.
It is therefore noticeable that β-Est is more highly expressed in S.
petrii than in S. sthenodactylus.