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Pearson, E.R., Velho, G., Clark, P., Stride, A., Shepherd, M., Frayling, T.M., Bulman, M.P., Ellard, S., Froguel, P. and Hattersley, A. (2001) β cell genes and diabetes: Quantitative and qualitative differences in the pathophysiology of hepatic nuclear factor 1α and glucokinase mutations. Diabetes, 50, S101-S107. http://dx.doi.org/10.2337/diabetes.50.2007.S101

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• TITLE: Analysis of the glucokinase gene in Iranian families with maturity onset diabetes of the young

  AUTHORS: Meisam Javadi, Houshang Rafatpanah, Seyed Morteza Taghavi, Jalil Tavakolafshari, Rashin Ganjali, Narges Valizadeh, Seyedeh Seddigheh Fatemi

  KEYWORDS: Maturity Onset Diabetes of the Young 2 (MODY2); Glucokinase (GCK); Mutation; SSCP; PCR

  JOURNAL NAME: Journal of Diabetes Mellitus, Vol.3 No.4, November 1, 2013

  ABSTRACT: Non insulin dependent diabetes mellitus (NIDDM) as a most common form of diabetes is a major public health problem; there is a subgroup of NIDDM patients who develop the disease at an early age and show a dominant mode of inheritance. This type is nominates Maturity onset diabetes of the young (MODY). The prevalence of MODY is difficult to access, and patients with MODY genes mutations are often identified during routine screening for other purposes. MODY2 was linked to glucokinase gene (GCK) mutations, and accounted for 8% to 56% of MODY, with the highest prevalence found in the southern Europe. The aim of this study was to examine the prevalence and nature of mutations in GCK gene in Iranian paients. We have screened GCK mutations by polymerase chain reaction (PCR); single stranded conformation polymorphism (SSCP) technique in 12 Iranian families with clinical diagnosis of MODY, included 30 patients (8 males and 22 females) and their 21 family members. PCR products with abnormal mobility in denaturing gradient gel electrophoresis (DGGE) were directly sequenced. We identified 6 novel mutations in GCK gene in Iranian families (corresponding to 36.6% prevalence). Our findings and the last study on MODY1 highlight that in addition to GCK, other MODY genes such as MODY3 and MODYX may play a significant role in diabetes characterized by monogenic autosomal dominant transmission. There is an important point that the genetic recognation can be used to pre-symptomatically identify family members at risk for developing MODY.