TITLE:
Molecular Cloning, Characterization and Expression Analysis of Macrohage-Colony Stimulating Factor 2 Gene from Grass Carp (Ctenopharyngodon idellus)
AUTHORS:
Linyong Du, Lei Qin, Shangnian Wang, Lu Yang, Kun Yang, Xinyan Wang, Hong Zhou
KEYWORDS:
Grass Carp; M-CSF2; Macrophages; Cloning; Sequence Analysis; Tissue Distribution
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
October
31,
2013
ABSTRACT: Macrophage colony-stimulating factor (CSF-1/M-CSF) is a key
factor for the differentiation, growth and survival of monocytes/macrophages and
osteoclasts. The functions of M-CSF have been well characterized in mammals. In
this study, we have cloned and sequenced the cDNA of M-CSF2 in grass carp. The grass
carp M-CSF2 cDNA was 1487 bp in length, containing an open reading frame of 855
bp that encodes 284 amino acids. The deduced protein of grass carp M-CSF2 possessed
same domains similar to its mammalian counterparts. Multiple alignments and phylogenetic
tree indicate that the grass carp M-CSF2 exhibits close evolutionary relationship
with its counterparts in other teleosts. Lastly, the tissue distribution results
also showed that the grass carp M-CSF2 transcript was dominantly expressed in head
kidney, kidney and spleen in vivo.