TITLE:
Human dermal reticular fibroblasts at confluence display a signature micro pattern in vitro
AUTHORS:
Denis E. Solomon
KEYWORDS:
Papillary and Reticular Fibroblasts; Cell Culture of Human Dermal Fibroblasts; Dermal Filler; Dermal Reticular Fibroblast Micro Pattern; Wound Healing; Anatomic Site Identity Code
JOURNAL NAME:
Open Journal of Regenerative Medicine,
Vol.2 No.4,
October
31,
2013
ABSTRACT: This paper sets out to
demonstrate that scraping of the flat dorsal surface of human dermis with a
scalpel blade and cell plating without centrifugation can lead to the
recognition and identification of the individual packing micro pattern of
dermal reticular fibroblasts at confluence. The characteristic alignment of
papillary and reticular fibroblasts at right angles to each other led to the
positive identification of reticular fibroblasts. A non-enzymatic means of
sub-culturing (passaging), which yields fully functional, healthy cells with
normal, phenotypic morphology is also described. Implications for published subcutaneous
wound healing studies are discussed as well as the confluent reticular
fibroblast configuration, interpreted as ananatomic site identity code,which may be the address of a specific fibroblast gene pattern expression.