TITLE:
Determination of Eight Sudan Dyes in Chili Powder by UPLC-MS/MS
AUTHORS:
Lin Wang, Jie Zheng, Zhe Zhang, Tiesong Wang, Baoquan Che
KEYWORDS:
Sudan Dyes; UPLC-MS/MS; Chili Powder
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
October
30,
2013
ABSTRACT: Sudan dyes are synthetic azo dyes which are widely used in industry.
Although they are not allowed in foodstuffs, they have been found
contaminating in different food products and their presence is regularly reported. It
is assumed that these appearances are due to cross-contamination or
adulteration. In this paper, we present a newly developed fast and sensitive
method for the quantification of eight Sudan dyes, using liquid-liquid
extraction and UPLC-MS/MS analysis. The calibration curves were linear over the
range of 0.1-25 mg/kg. Mean recovery for the eight Sudan dyes ranged from
80.7% to 104.4%, and with the inter-day and intra-day precisions
ranged from 2.24% to 12.2%. The method was successfully applied in the determination
of Sudan dyes in chili powder of 10 samples.