TITLE:
Neuroprotective Effects of Ginkgo Biloba Extract (GbE) on Oxygen-Glucose Deprivation (OGD) in PC12 Cells
AUTHORS:
Chunli Mei, Xuelu Han, Jing Zhang, Ling Gao, Huimin Liu
KEYWORDS:
NGF; OGD; PC12 Cells; Ginkgo Biloba; Neuroprotective Effects
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
October
30,
2013
ABSTRACT: Ischemic cerebrovascular disease is a global health
problem. According to the World Health Organization, ischemic stroke is
actually the most common cause of death in the world. Ginkgo biloba extract
(GbE) is a traditional Chinese medicine for angina pectoris. Ginkgo biloba
plays a role in expanding blood vessels, increasing coronary and cerebral blood
flow, preventing platelet aggregation, inhibiting thrombosis, and improving the
microcirculation. In the present study, we investigated the mechanisms involved
in the neuroprotective effects of GbE in a model of hypoxic-ischemic brain
disease. We used NGF(100 ng/ml for 6 days)and OGD(5% CO2and 95% N2, 1 mmol/l NaS2O4insugar-free DMEM for 16 h) to stimulate
PC12 cells and convert them into neurons in order to establish an ischemia
model. The results showed that PC12 cells transformed into cells that looked
like neurons and that MAP2 was up-regulated in NGF-treated PC12 cells. Cell
apoptosis was found to be up-regulated after NGF stimulation and OGD. The
apoptosis rate after 16 hours of OGD was 19.44%. GbE (50ng/ml) reduces
apoptosis rate to 11.35%. These results may help to show that NGF treatment can
be combined with OGD to establish anin
vitromodel of acute ischemic brain damage. In the present study, we find that
GbE effectively increases the survival rate of PC12 cells and relieves OGD
damage. These results suggest that GbE has the neuroprotective effects of
ischemic brain damage.