Article citationsMore>>

K. Ishii, Y. Hirano, N. Araki, T. Oda, M. Kumeta, K. Takeyasu, et al., “Nuclear Matrix Contains Novel WDRepeat and Disordered-Region-Rich Proteins,” FEBS Letters, Vol. 582, No. 23-24, 2008, pp. 3515-3519.

has been cited by the following article:

• TITLE: AFM Investigation of the Organization of Actin Bundles Formed by Actin-Binding Proteins

  AUTHORS: Jamie L. Gilmore, Masahiro Kumeta, Kunio Takeyasu

  KEYWORDS: F-Actin; Caprice; α-Actinin

  JOURNAL NAME: Journal of Surface Engineered Materials and Advanced Technology, Vol.3 No.4A, October 22, 2013

  ABSTRACT: AFM is a powerful technique for revealing the morphological features of various biological systems at high resolution. However, one of the complications of AFM is that samples must be attached to a flat surface in order to obtain images. This often requires the development of specialized methods depending on the sample which is being used. In this study, we developed a novel technique to image actin bundles on the mica surface. Using this technique, we were able to image molecular assemblies of F-actin with two actin remodeling proteins: α-actinin and Caprice. High resolution AFM images of F-actin fibers and bundle organization depicted two different types of molecular assemblies: F-actin bundles forming an elongated “zipper” structure in the presence of α-actinin, and bundles forming a perpendicularly crossing the mesh structure in the presence of Caprice.