TITLE:
Preparation of Water Soluble Yeast Glucan by Four Kinds of Solubilizing Processes
AUTHORS:
Liping Du, Xuekuang Zhang, Chao Wang, Dongguang Xiao
KEYWORDS:
Glucan; Solubility; FT-IR Spectra; 13C NMR Spectra; Immunoprophylaxis Efficacy
JOURNAL NAME:
Engineering,
Vol.4 No.10B,
October
21,
2013
ABSTRACT: (1→3)-β-D-glucanfrom the inner cell wall of
Saccharomyces cerevisiae is considered a member of a class of drugs known as biological
response modifiers (BRM). However the glucan was an insoluble polysaccharide,
which could be the major barrier to the utilization of glucan. In this case,
the insoluble glucan was convent into a soluble form by four kind of
solubilizing processes. The yield, solubility, chemistry structure and
immunoprophylaxis efficacy of the soluble products were compared. Our date
suggest that urea has a significant effect on yield, and DMSO has a significant
effect on solubility. FT-IR spectra, 13C
NMR spectra and helix-coil transition analysis demonstrate that the chemistry
structure of native and solubilizing glucans have no significant difference.
They still have the triple helical structure. The solubility and
immunoprophylaxis efficacy assay indicate that the introduction of phosphate
group not only enhanced the solubility of glucan, but also improved the
survival rate of mice challenged with E.
coli.