TITLE:
Antitumor Effects of Conditional Replication Adenovirus in Combination with Cisplatin on Lung Cancer
AUTHORS:
Yanan Liu, Yinghui Huang
KEYWORDS:
Lung Cancer; Adenovirus; Cisplatin
JOURNAL NAME:
Engineering,
Vol.4 No.10B,
October
21,
2013
ABSTRACT: Object:
To explore the therapeutic effects and therapeutic mechanisms of Conditional
Replication Adenovirus(CRAd) in combination with cisplatin on lung cancer
cells. Methods: Using MTS / PMS assay, in
vitro cell inhibition assay was performed to detect the cell viability of
two lung cancer cell lines, NCI-H292 and NCI-H661. PCR was employed to detect
the Coxsackie receptor(CAR) expression of cancer cells. The in vivo anti-tumor effect of CRAd and
cisplatin was evaluated using a subcutaneous mouse model. Results: The CRAd
with cisplatin is superior to the use of cisplatin or CRAd viruse alone on the
suppression of lung cancer cell growth. The mechanism of inhibition is
associated with the increased CAR expression. Conclusion: The application of
CRAd in combination with cisplatin could play a better therapeutic effect on
lung cancer cell growth inhibition.