TITLE:
The omp2 Gene of HPS-type Bacteria Cloning and Sequence Analysis Isolates from Sichuan Province
AUTHORS:
Lirui Li, Zexiao Yang, Yin Wang, Qiu Jin, Xulong Wu, Rongchang Zhang
KEYWORDS:
Haemophilus parasuis, virulence genes, omp2
JOURNAL NAME:
Engineering,
Vol.4 No.10B,
October
21,
2013
ABSTRACT: In order to compare the
homology and antigen of Haemophilus parasuis (HPS)4 outer membrane protein P2(omp2), we design a test with specific primers, using PCR amplification of isolates of Haemophilus parasuis from Sichuan Province(HP
Sch2010), ompP2 gene will be cloned into the pGM-T vector, and transformed into
E. coli DH5α. Identified by PCR and sequencing and analysis, the sequencing
results showed that the published 4 HPS SW124 strains omp2 gene (1077bp), compared with the amplified 1086bp purpose
fragment(containing omp2 genes), is relatively stable, with the nucleotide
homology level 97% and amino acid homology level of 92.5%. The
variable regions are mainly concentrated in the three base sequences:
40-65,110-156,180-202.