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V.A de la Puente Redondo,J Navas Méndez,N Garc??a del Blanco,N Ladrón Boronat,C.B Gutiérrez Mart??na, E.F Rodr??guez Ferri.Typing of Haemophilus parasuis strains by PCR-RFLP analysis of the tbpA gene.Vet Microbiol. vol..92, pp.253-262, 2003．

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• TITLE: The omp2 Gene of HPS-type Bacteria Cloning and Sequence Analysis Isolates from Sichuan Province

  AUTHORS: Lirui Li, Zexiao Yang, Yin Wang, Qiu Jin, Xulong Wu, Rongchang Zhang

  KEYWORDS: Haemophilus parasuis, virulence genes, omp2

  JOURNAL NAME: Engineering, Vol.4 No.10B, October 21, 2013

  ABSTRACT: In order to compare the homology and antigen of Haemophilus parasuis (HPS)4 outer membrane protein P2(omp2), we design a test with specific primers, using PCR amplification of isolates of Haemophilus parasuis from Sichuan Province(HP Sch2010), ompP2 gene will be cloned into the pGM-T vector, and transformed into E. coli DH5α. Identified by PCR and sequencing and analysis, the sequencing results showed that the published 4 HPS SW124 strains omp2 gene (1077bp), compared with the amplified 1086bp purpose fragment(containing omp2 genes), is relatively stable, with the nucleotide homology level 97% and amino acid homology level of 92.5%. The variable regions are mainly concentrated in the three base sequences: 40-65,110-156,180-202.