TITLE:
Effects of Storage Duration and Temperature of Human Plasma and Serum on Red Blood Cell Aggregation and Shape
AUTHORS:
Yury A. Sheremet’ev, Alexandra N. Popovicheva, Gregory Ya. Levin
KEYWORDS:
In Vitro Time; Plasma and Serum Storage; Temperature; RBC; Aggregation; Cell Shape
JOURNAL NAME:
Open Journal of Biophysics,
Vol.3 No.4,
October
17,
2013
ABSTRACT: Platelet-free plasma of human blood (sodium citrate and EDTA as an
anticoagulant) and serum were stored at 4°C, room temperature (25°C) and at
37°C for 24 hours. RBC aggregation decreased after incubation of plasma and
serum at 37°C for 4 hours. The RBC shape was changed at the same time:
discocytes transformed to echinocytes. Storage of plasma and serum at 4°C and
room temperature did not lead to significant alterations of RBC aggregation. The RBC
shape did not change in influence of such plasma and serum. The most considerable decrease
of RBC aggregation and change of their shapes were observed in
the plasma and serum incubated at 37°C for 24 hours. Dilution of incubated
plasma by fresh plasma led to consistent restoration of erythrocyte shape and
their aggregation.