TITLE:
Characterization of purified β-glucosidase produced from Trichoderma viride through bio-processing of orange peel waste
AUTHORS:
Muhammad Irshad, Zahid Anwar, Muhammad Ramzan, Zahed Mahmood, Haq Nawaz
KEYWORDS:
Orange Peel Waste; β-Glucosidase; T. viride; Purification; SDS-PAGE; Characterization
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.10,
October
16,
2013
ABSTRACT: In the present study, solid state fermentation was carried out using
orange peel waste to produce β-glucosidase from Trichoderma viride. A locally isolated fungal strain T. viride was cultured in the solid state medium of orange peel (50% w/w
moisture) under optimized fermentation conditions and maximum activity of 515
± 12.4 U/mL was recorded after 4th day of incubation at pH 5.5 and
30℃.
Indigenously produced β-glucosidase
was subjected to the ammonium sulfate precipitation and
Sephadex-G-100 gel filtration chromatography. In comparison to the crude extract β-glucosidase was 5.1-fold purified with specific
activity of 758 U/mg. The enzyme was shown to have a relative molecular weight
of 62 kDa as evidenced by sodium dodecyl sulphate polyacrylamide gel electrophoresis.
The purified β-glucosidase displayed
6 and 60℃ as an optimum pH and temperature respectively.