TITLE:
Adsorption of α-Chymotrypsin on Plant Biomass Charcoal
AUTHORS:
Hidetaka Noritomi, Keito Hishinuma, Shunichi Kurihara, Jumpei Nishigami, Tetsuya Takemoto, Nobuyuki Endo, Satoru Kato
KEYWORDS:
Adsorption; Characterization; Plant Biomass Charcoal; α-Chymotrypsin; Protein
JOURNAL NAME:
Journal of Surface Engineered Materials and Advanced Technology,
Vol.3 No.4,
October
10,
2013
ABSTRACT: The adsorption of α-chymotrypsin onto plant biomass charcoal (PBC), which was
prepared from plant biomass wastes such as bagasse and dumped adzuki beans by
pyrolysis, has been examined. The PBC was characterized by SEM, specific
surface area, and pore size distribution. The adsorption isotherms were
successfully correlated by the Freundlich equation. The amount of α-chymotrypsin adsorbed on PBC was
dramatically dependent upon the solution pH and temperature. Maximum
adsorptions of α-chymotrypsin on
adzuki bean charcoal and bagasse charcoal were observed at weak acidic and near
neutral pH, respectively. The amount of α-chymotrypsin
adsorbed on PBC decreased with an increase in the concentration of salts. Plots
of the amount of α-chymotrypsin adsorbed
on PBC versus temperature exhibited an optimum temperature.