TITLE:
In Vitro Plant Regeneration of Morus indica L. cv. V1 Using Leaf Explant
AUTHORS:
Melur Kodandaram Raghunath, Karaba N. Nataraja, Jyothi Sainath Meghana, Radha Sivarajan Sajeevan, Mala V. Rajan, Syed Mashayak Hussaine Qadri
KEYWORDS:
In Vitro Regeneration; Adventitious Bud; Thidiazuron; Mulberry cv. V1; Carbon Source
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.10,
October
9,
2013
ABSTRACT: Adventitious bud induction and plantlet regeneration were studied in a popular
mulberry variety, V1 using leaf as an explant. Fully expanded leaf explants
were cultured on Murashige and Skoog’s (MS) medium supplemented with thidiazuron (TDZ)
(0.5-4.0 mg/l), 6-benzylaminopurine (BAP) (0.5-2.0 mg/l), indole acetic
acid (IAA) (2.0 mg/l), gibberlic acid (GA3) (1.0-2.0
mg/l) silver nitrate (AgNO3) (2.0 mg/l) and different carbon sources
such as sucrose, fructose and glucose (10%-30%) either individually
or in combination to induce adventitious buds and regeneration. The highest
percentage (63%) of adventitious bud formation and regeneration (68%) was achieved in the medium
containing MS with TDZ (1.0 mg/l), IAA (2.0 mg/l) and AgNO3 (2.0
mg/l). For subsequent regeneration and shoot elongation the MS medium having
BAP (1.0 mg/l), GA3 (2.0 mg/l) and AgNO3 (2.0 mg/l) was found to be
suitable. Amongst the carbon sources tested, the most suitable carbon source
was found to be sucrose (3%) followed by fructose (2%) for adventitious bud
formation. Excised in vitro shoots
were rooted (60%-80%) in half strength MS medium supplemented with indole-3-butyric
acid (1.0 mg/l). The well rooted plantlets were hardened in soil + sand + farm
yard manure (FYM) mixture with a success rate of 70%-90%. Since in vitro regeneration is highly genotype-dependent in mulberry, the
standardized protocol can be effectively used for further improvement of this
leading genotype using biotechnological approaches.