TITLE:
Mesenchymal stem cell enhances chondral defects healing in horses
AUTHORS:
Ana Lucia Miluzzi Yamada, Armando de Mattos Carvalho, Andrei Moroz, Elenice Deffune, Marcos Jun Watanabe, Carlos Alberto Hussni, Celso Antônio Rodrigues, Ana Liz Garcia Alves
KEYWORDS:
Arthroscopy; Cartilage; Equine; Lameness; Mesenchymal Stem Cells
JOURNAL NAME:
Stem Cell Discovery,
Vol.3 No.4,
October
8,
2013
ABSTRACT: The purpose of this
study was to evaluate the effect of intralesional Mesenchymal Stem Cells (MSC)
on the treatment of experimentally induced articular chondral defects in
horses, emphasizing the benefits of this application in veterinary medicine.
Chondral defects were induced in the medial femoral trochlea of both hind limbs
of four horses. Thirty days post induction; the horses were divided into two
groups. The G1 was submitted to treatment with MSC and the G2 was the control
group. Clinical
evaluations, synovial fluid analysis and synovial Prostaglandin E2 (PGE2) assessment were performed prior to defects and fortnightly up
to 120 and 150 days. Macroscopic, histopathological and histochemical
evaluations were performed at the end of the experiment. The treatment with MSC
reduced the intraarticular inflammatory process. The G1 showed lower PGE2 concentrations in the synovial fluid and greater percentage of mononuclear
cells and lower percentages of lymphocytes and neutrophils. The treatment
improved the macro and microscopic aspects of repair tissue. No difference was
observed in the scores of lameness between the G1 and G2. The use of MSC
in the treatment of chondral defects minimized joint inflammation, as confirmed
by synovial fluid analysis. The treatment resulted in an improved repair tissue, verified by macroscopic examination, histochemical and histopathological
analysis.