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Liu, G., Iwata, K., Ogasawara, T., Watanabe, J., Fukazawa, K., Ishihara, K., Asawa Y., Fujihara, Y., Chung, U.L., Moro, T., Takatori, Y., Takato, T., Nakamura, K., Kawaguchi, H. and Hoshi, K. (2010) Selection of highly osteogenic and chondrogenic cells from bone marrow stromal cells in biocompatible polymer-coated plates. Journal of Biomedical Materials Research Part A, 92, 1273-1282. http://dx.doi.org/10.1002/jbm.a.32460

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• TITLE: Evaluation of in vivo migration of chondrocytes from tissue-engineered cartilage that was subcutaneously transplanted in mouse model

  AUTHORS: Mariko Matsuyama, Yuko Fujihara, Ryoko Inaki, Satoru Nishizawa, Satoru Nagata, Tsuyoshi Takato, Kazuto Hoshi

  KEYWORDS: Cartilage; Tissue Engineering;Chondrocyte; Migration

  JOURNAL NAME: Open Journal of Regenerative Medicine, Vol.2 No.4, October 7, 2013

  ABSTRACT: For regenerative medicine, clarification of in vivo migration of transplanted cells is an important task to secure the safety of transplanted tissue. We had prepared tissue-engineered cartilage consisting of cultured chondrocytes with collagen hydrogel and a biodegradable porous polymer, and we clinically applied it for treatment of craniofacial anomaly. To verify the safety of this tissue-engineered cartilage, we had syngenically transplanted the tissue-engineered cartilage using chondrocytes harvested from EGFP-transgenic mice into subcutaneous pocket of wild type mice, and investigated localizations of transplanted chondrocytes in various organs including cerebrum, lung, liver, spleen, kidney, auricle, gastrocnemius, and femur. After 8 to 24 weeks of the transplantation, accumulation of cartilaginous matrices was observed in tissue-engineered cartilage, while EGFP-positive transplanted chondrocytes were localized in this area. Otherwise, no EGFP was immunohistochemically detected in each organ, suggesting that subcutaneously-transplanted chondrocytes do not migrate to other organs through the circulation. In cartilage tissue engineering using cultured chondrocytes, risk for migration and circulation of transplanted cells seemed negligible, and that ectopic growth of the cells was unlikely to occur, showing that this is safe technique with regard to the in vivo migration of transplanted cells.