TITLE:
Estimation of Vitamins B-Complex (B2, B3, B5 and B6) of Some Leafy Vegetables Indigenous to Bangladesh by HPLC Method
AUTHORS:
Md. Nazmul Hasan, M. Akhtaruzzaman, Md. Zakir Sultan
KEYWORDS:
HPLC Analysis; Vitamin B2; Vitamin B3; Vitamin B5; Vitamin B6; Leafy Vegetables
JOURNAL NAME:
Journal of Analytical Sciences, Methods and Instrumentation,
Vol.3 No.3A,
September
26,
2013
ABSTRACT: The current experiment was conducted
for the simultaneous determination of several water-soluble vitamins like ribo- flavin (vitamin B2), niacin
(vitamin B3), pantothenic acid (vitamin B5) and
pyridoxine (vitamin B6) in five highly con- sumed local leafy vegetables named as
bottle gourd leaves (Lagenaria vulgaris) (local name Lau shak), green
amaranth leaves (Amaranthus viridis)
(local name Data shak), red amaranth leaves (Amaranthus gangeticuss)
(local name Lal shak), Indian spinach (Basella alba) (local name Pui
shak) and bitter gourd leaves (Momordica charantia) (local name Korola
shak). The analyses were performed by HPLC using an analytical reversed phase
C-18 (ODS column, 250 × 4.6 mm, 5 μm, Phenomenex, Inc.) column with the mobile
phase consisting of a mixture of buffer (hexane sulphonic acid sodium,
potassium dihydrogen phosphate and triethylamine, pH 3.0) and methanol in the
ratio of 96:4 (v/v) at a flow rate of 1 mL/min with UV detection at 210 nm. The
retention times for the vitamins were obtained as 3.61 min, 6.37 min, 9.51 min
and 11.51 min for Vitamins B2, B3, B5 and B6,
respectively. These obtained values of the vitamins were compared with the
values available in published literatures of Deshio Khaddar Pustiman (DKPM), Indian food value (IFV) and United States
Department of Agriculture (USDA).