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V. G. Sharov, N. I. Afonskaya, M. Y. Ruda, N. M. Cherpachenko, EYa Pozin, R. A. Markosyan, II Shepeleva, M. B. Samarenko and V. A. Saks, “Protection of Ischemic Myocardium by Exogenous Phosphocreatine (Neoton): Pharmacokinetics of Phosphocreatine, Reduction of Infarct Size, Stabilization of Sarcolemma of Ischemic Cardiomyocytes, and Antithrombotic Action,” Biochemical Medicine and Metabolic Biology, Vol. 35, No. 1, 1986, pp. 101-114. http://dx.doi.org/10.1016/0885-4505(86)90064-2

has been cited by the following article:

• TITLE: An Ion-Pair HPLC Method for Simultaneous Determination of Exogenous Phosphocreatine and Its Metabolite Creatine and Related ATP in Rabbit Plasma and RBC: Application to a Pharmacokinetic Study

  AUTHORS: Li Lv, Heng Xi, Guozhu Han

  KEYWORDS: Ion-Pair HPLC; Phosphocreatine; Creatine; ATP; Plasma; RBC; Pharmacokinetics; Rabbit

  JOURNAL NAME: Journal of Analytical Sciences, Methods and Instrumentation, Vol.3 No.3A, September 26, 2013

  ABSTRACT: A specific, precise and accurate ion-pair HPLC-UV method has been developed and validated for simultaneous determination of phosphocreatine (PCr), and its metabolite creatine (Cr) as well as related ATP in plasma and red blood cell (RBC) of rabbits. After addition of TMP as IS, the samples were deproteinized with 6% PCA. The analytes were separated on a Kromasil C18 column using a tertiary gradient mobile phase composed of buffer A (0.2% KH2PO4 + 0.08% tetrabutyl ammonium hydrogen sulphate, pH 3.0), buffer B (buffer A adjusted to pH 7.5 with 1 mol/L NaOH) and MeOH. Detection wavelengths were set at 210 nm for PCr and Cr and 260 nm for ATP and TMP. Some blank samples were initially run for baseline subtraction. The linear detection responses were obtained for PCr concentration over a range of 10 - 7500 mg/ml (plasma) and 5 - 2500 mg/ml (RBC) and for both Cr and ATP concentrations of 10 - 1500 mg/ml (plasma) and 5 - 750 mg/ml (RBC) (r > 0.99). The QC samples of 3 analytes showed intra-day and inter-day precisions (RSD) of - 107%. The method was successfully used to simultaneously determine plasma and RBC concentrations of the 3 analytes and to study pharmacokinetics after iv administration of PCr to rabbits.