TITLE:
Cytokine gene expression in human hepatocytes infected with dengue virus serotype 3 (strain-16562)
AUTHORS:
Sutee Yoksan, Jundee Rabablert, Kumchol Chaiyo, Supoth Rajchakam, Supathra Tiewcharoen, Natthapol Rabablert, Soratorn Kerdkriangkrai, Narong Samngamnim, Watchara Phurttikul, Tarmphong Luangboribun
KEYWORDS:
Dengue Virus; Hepatocyte; Cytokines; Type I Interferon; Pattern Recognition Receptors
JOURNAL NAME:
Health,
Vol.5 No.9,
September
23,
2013
ABSTRACT: Liver is a site of viral replication and liver dysfunction
is a characteristic of severe dengue infection. To understand these
mechanisms, we analyzed the response of a hepatic cell linage, HepG2 to
infection with dengue 3 virus (strain 16562). Steady state levels of mRNA accumulation were assessed for 14 genes involved in modulation of the host immune
responses, at 6, 24 and 48 hpi, by quantitative reverse transcription real-time
PCR (qRT-PCR). Fourteen genes showed altered expression upon infection
with D3V including; cytokines/chemokines (IL-1β, IL-6, IL-8, RANTES, MCP-2, IL-2Rα and TGF-βIIIR), type I
interferon (IFN-α and IFN-β), and pattern-recognition receptors
(TLR3, TLR8, RIG-1, MDA5 and MyD88). Although these genes are associated with
mechanism of innate immune response and anti-viral activity, their altered expression
does not inhibit D3V (strain 16562) growth
kinetics and virus yield in HepG2 cells. Gene expression in liver
may explain pathological changes associated with dengue virus infection.