TITLE:
Changes in the Nuclei of Infected Cells at Early Stages of Infection with EMCV
AUTHORS:
Zaven A. Karalyan, Hranush R. Avagyan, Hovakim S. Zakaryan, Liana O. Abroyan, Lina H. Hakobyan, Aida S. Avetisyan, Elena M. Karalova
KEYWORDS:
EMCV; Acidic Proteins; RNA; Nucleolus; Nucleus
JOURNAL NAME:
CellBio,
Vol.2 No.3,
September
18,
2013
ABSTRACT: By the methods of quantitative cytophotometry, we have
identified the changes in the nucleus and of some intranuclear compartments in
the early stages of infection with encephalomyocarditis virus (EMCV). They can
be characterized as early 1 - 2 hours post infection (hpi) and temporary
increase (duration about 1 hour) in the content of the acidic proteins of the
nucleolus, changing their decline to the control values. Then (after 1 - 2 hours)
follows an increase in RNA content of nucleoli to 4 hours post infection (the
process takes about 2 hours). The increase in RNA content in nucleoli is in
approximately the same time (slightly behind) with the activation of PML bodies
(2 - 4 hpi). Then, the RNA content in nucleoli decreased to the control
values, while simultaneously decreasing activity of PML bodies (ranging from 5 - 6 hpi).
The early stages of infection EMCV are also characterized by the tendency to
increase in the size of the nuclei of infected cells, and preserve at a later
time. Then there is an increase in RNA content in the nucleus, roughly coinciding
with the increased content of RNA in the nucleoli.