TITLE:
Generation of Unfolded DNA in Human Neutrophils Following Hypothermal Treatment
AUTHORS:
Jin Kawata, Makoto Kikuchi, Hisato Saitoh
KEYWORDS:
Hypothermal Treatment; DNA Unfolding; Neutrophil Extracellular Trap (NET)
JOURNAL NAME:
CellBio,
Vol.2 No.3,
September
18,
2013
ABSTRACT: By visualizing DNA with diamidino phenylindole (DAPI),
we found that hypothermal incubation followed by rewarming of human
neutrophils resulted in an increased number of DAPI-positive objects
representative of extensive DNA unfolding seemingly similar to neutrophil
extracellular traps (NETs). In contrast
to canonical NET formation, diphenylene iodonium (DPI), an NADPH oxidase inhibitor, exhibited negligible effects on formation of the
DAPI-positive objects. Moreover, multiple instances of DNA damage were detected in
the objects, but not in canonical NETs. Our results
thus suggest the potential of hypothermia for triggering DNA structural
alteration in neutrophils, which is similar to but distinct from NET formation.