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W. M. Merritt, A. A. Kamat, J. Y. Hwang, J. BottsfordMiller, C. Lu, Y. G. Lin, D. Coffey, W. A. Spannuth, E. Nugent, L. Y. Han, C. N. Landen, A. M. Nick, R. L. Stone, K. Coffman, E. Bruckheimer, R. R. Broaddus, D. M. Gershenson, R. L. Coleman and A. K. Sood, “Clinical and Biological Impact of EphA2 Overexpression and Angiogenesis in Endometrial Cancer,” Cancer Biological Therapy, Vol. 10, No. 12, 2010, pp. 1306-1314. http://dx.doi.org/10.4161/cbt.10.12.13582

has been cited by the following article:

• TITLE: Usefulness of Immuno-Magnetic Beads Conjugated with Anti-EpCAM Antibody for Detecting Endometrial Cancer Cells

  AUTHORS: Yoshikatsu Koga, Satoshi Katayose, Nobuko Onoda, Takahiro Kasamatsu, Tomoyasu Kato, Shunichi Ikeda, Mitsuya Ishikawa, Ken Ishitani, Yasuo Hirai, Hideo Matsui, Yasuhiro Matsumura

  KEYWORDS: Immuno-Magnetic Beads; Auto-Fluorescence; Endometrial Cancer; Cancer Screening

  JOURNAL NAME: Journal of Cancer Therapy, Vol.4 No.8, September 17, 2013

  ABSTRACT: A simple and non-invasive method for detecting endometrial cancer in women with abnormal uterine bleeding is required. For this purpose, we prepared immuno-magnetic beads conjugated with anti-human EpCAM rat monoclonal antibody (mAb) for isolating exfoliated endometrial cells including endometrial cancer cells in vaginal discharge. The affinities of the anti-human EpCAM rat mAbs were analyzed by flow cytometry and immunocytochemistry and then magnetic beads were conjugated with the mAbs. The rate of retrieval of endometrial cells using the immuno-magnetic beads was calculated. Endometrial cells were isolated using the immuno-magnetic beads from the vaginal discharges of 22 patients with endometrial cancer and 16 non-malignant controls. The isolated cells were stained using endometrial cancer specific-mAbs and analyzed by flow cytometry and imaging cytometry. The immuno-magnetic beads conjugated with high-affinity mAb (clone 1456) appeared to have very low auto-fluorescence. Sufficient enrichment of Ep-CAMpositive cells using immuno-magnetic beads was observed in both simulation and clinical samples. The overall sensitivities of flow cytometry and imaging cytometry to detect endometrial cancer cells were 72.7% and 45.5%, respectively. Meanwhile, the overall specificities of flow cytometry and imaging cytometry for healthy controls were 75.0% and 81.3%, respectively. Our immuno-magnetic beads have very low auto-fluorescence, so they could be useful for fluorescent analysis, such as fluorescent immunochemical staining. In the future, these novel immuno-magnetic beads could be used for cytological study.