TITLE:
Separation of Inorganic Anions Using Methacrylate-Based Monolithic Column Modified with Trimethylamine in Ion Chromatography Capillary System
AUTHORS:
Fitri Mairizki, Athika Rahmah, Hilma, Radhia Putri, Rahmiana Zein, Lee Wah Lim, Toyohide Takeuchi, Edison Munaf
KEYWORDS:
Methacrylate-Based Monolithic Column; Trimethylamine; Ion Chromatography Capillary System; Inorganic Anions; Water Sample
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.9,
September
13,
2013
ABSTRACT: Methacrylate-based monolithic column was prepared in
fused-silica capillary (80 ′ 0.32 mm i.d.) by in situ polymerizetion
reaction using glycidyl methacrylate
as monomer; ethylene dimethacrylate as crosslinker; 1-propanol, 1,4-butanediol,
and water as porogenic solvents. The monolith matrix was modified with
trimethylamine to create strong anion exchanger via ring opening reaction of
epoxy groups. The morphology of the monolithic column was studied by using Scanning Electron Microscope (SEM). This
column had good mechanical stability and permeability. The effects of
various mobile phases for separation of inorganic anions were investigated.
Iodate, bromate, nitrite, bromide, and nitrate were separated within 11 min
using100 mMpotassium chloride as mobile phase and detected at 210 nm. This method showed
good precision of retention time, acceptable linearity and good sensitivity.
Under the optimum condition, the RSD of the retention time was in the range of 1.09%-1.75% (n = 6). The
calibration curve showed linear relationships between the peak area and the
concentration. The limits of detection (LOD) and the limits of quantitation
(LOQ) were between 0.08-0.18 mM and 0.26-0.61 mM, respectively. This method was applied to the determination of inorganic
anions in tap water and ground water samples.