TITLE:
A Novel in Vitro Three-Dimensional Macroporous Scaffolds from Bacterial Cellulose for Culture of Breast Cancer Cells
AUTHORS:
Guangyao Xiong, Honglin Luo, Feng Gu, Jing Zhang, Da Hu, Yizao Wan
KEYWORDS:
3D Culture; Scaffold; Bacterial Cellulose; Cancer Cell; Macropore
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.4 No.4,
September
11,
2013
ABSTRACT: In this work, patterned macropores
with a diameter larger
than 100 μm were introduced to pristine three-dimensional (3D) nanofibrous
bacterial cellulose (BC) scaffolds by using the infrared laser micromachining technique in an attempt to create an in vitro model for the culture of breast cancer cells. The
morphology, pore structure, and mechanical performance of the obtained patterned
macroporous BC (PM-BC) scaffolds were characterized by scanning electron microscopy (SEM), mercury intrusion
porosimeter, and mechanical testing. A human breast cancer cell (MDA-MB-231)
line was cultured onto the PM-BC scaffolds to investigate the role of
macropores in the control of cancer cell behavior. MTT assay, SEM, and
hematoxylin and eosin (H&E) staining were employed to determine cell
adhesion, growth, proliferation,
and infiltration. The PM-BC scaffolds were found to be able to promote cellular
adhesion and proliferation on the scaffolds, and further to allow for cell
infiltration into the PM-BC scaffolds. The results demonstrated that BC scaffolds with laser-patterned macropores
were promising for the in vitro 3D
culture of breast cancer cells.