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Ishibashi, K., Kuwahara, M., Gu, Y., Kageyama, Y., Tohsaka, A., Suzuki, F., Marumo, F. and Sasaki, S. (1997) Cloning and functional expression of a new water channel abundantly expressed in the testis permeable to water, glycerol and urea. Journal Biological Chemistry, 272, 20782-20786. doi:10.1074/jbc.272.33.20782

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• TITLE: Enhanced aquaporin 8 expression after subtotal colectomy in rat

  AUTHORS: Masato Nakano, Yu Koyama, Hitoshi Nogami, Tadashi Yamamoto, Toshifumi Wakai

  KEYWORDS: Aquaporin 8; Subtotal Colectomy; mRNA; Immunohistochemistry

  JOURNAL NAME: Open Journal of Gastroenterology, Vol.3 No.5, September 6, 2013

  ABSTRACT: Background: Aquaporins (AQPs), the family of water-selective channels, are localized in various organs and tissues, including the gastrointestinal (GI) tract. However, the roles of AQPs in the GI tract remain unclear. Materials and Methods: Male SD rats were subjected to subtotal colectomy (Group C, n = 22) or a sham operation (Group S, n = 16) and were sacri-ficed on postoperative days 7, 14, and 28. Total RNAs from the distal ileum and rectum were extracted. Quantitative RT-PCR was performed to measure AQP8 mRNA expression. For light-microscopy or immunohistochemistry, paraffin-embedded sections of 4 μm were prepared with H-E staining or anti-AQP8 antibody reaction. Mann-Whitney U-test was performed to compare the AQP8 distributions between the two groups, and the statistical significance was defined as p