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Jung, J.S., Bhat, R.V., Preston, G.M., Guggino, W.B., Baraban, J.M. and Agre, P. (1994) Molecular characterization of an aquaporin cDNA from brain candidate osmoreceptor and regulator of water balance. Proceeding of the National Academy of Sciences of the United States of America, 91, 13052-13056.

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• TITLE: Enhanced aquaporin 8 expression after subtotal colectomy in rat

  AUTHORS: Masato Nakano, Yu Koyama, Hitoshi Nogami, Tadashi Yamamoto, Toshifumi Wakai

  KEYWORDS: Aquaporin 8; Subtotal Colectomy; mRNA; Immunohistochemistry

  JOURNAL NAME: Open Journal of Gastroenterology, Vol.3 No.5, September 6, 2013

  ABSTRACT: Background: Aquaporins (AQPs), the family of water-selective channels, are localized in various organs and tissues, including the gastrointestinal (GI) tract. However, the roles of AQPs in the GI tract remain unclear. Materials and Methods: Male SD rats were subjected to subtotal colectomy (Group C, n = 22) or a sham operation (Group S, n = 16) and were sacri-ficed on postoperative days 7, 14, and 28. Total RNAs from the distal ileum and rectum were extracted. Quantitative RT-PCR was performed to measure AQP8 mRNA expression. For light-microscopy or immunohistochemistry, paraffin-embedded sections of 4 μm were prepared with H-E staining or anti-AQP8 antibody reaction. Mann-Whitney U-test was performed to compare the AQP8 distributions between the two groups, and the statistical significance was defined as p