TITLE:
Rolling Circle Amplification on Biotin-Streptavidin Complexes Immobilized to Activated Cyclic Polyolefin Surfaces
AUTHORS:
Herin Oh, Alec Cerchiari, Desirée Sørensen, Timothy Mon, Cassandra L. Smith
KEYWORDS:
Cyclic Polyolefin; Ligation Mediated Rolling Circle Amplification; SNP Detection
JOURNAL NAME:
Materials Sciences and Applications,
Vol.4 No.9,
September
6,
2013
ABSTRACT: Cyclic polyolefin (COP) is an inexpensive hydrophobic material with low auto-fluorescence, high light transmittance and thermal stability, broad chemical resistance and no non-specific protein binding. Here, the hydrophobic alkane COP was modified to have carbonyl functionalities through oxygen plasma and chemical etching treatments to increase usefulness for chemical and biochemical applications. Then, biotin-hydrazide was used to create biotinylated surfaces that bound streptavidin. A biotinylated target oligonucleotide was subsequently bound to the immobilized biotin-streptavidin and ligation mediated rolling circle amplification-based (L-RCA) SNP detection was demonstrated.