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Shao-Hua Chiou, Wen-Teng Wu. “Immobilization of Candida rugosa Lipase on Chitosan with Activation of the Hydroxyl Groups,” Biomaterials, Vol. 25, No. 2, 2004, pp. 197-204. doi:10.1016/S0142-9612(03)00482-4

has been cited by the following article:

• TITLE: New Colorimetric Method for Lipases Activity Assay in Microbial Media

  AUTHORS: Mohamed A. Abd-Elhakeem, Ahmed M. Elsayed, Taher A. Alkhulaqi

  KEYWORDS: Lipase; Colorimetric Method; Assay; Phenyl Acetate; Folin

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.4 No.9, September 4, 2013

  ABSTRACT: A simple, rapid and precise method has been developed for determination of lipase activity in microbial media. The method is based on using phenyl acetate as substrate for lipase and determination of liberated phenol by Folin Ciocalteu reagent. Reaction mixture containing substrate 2.4 ml of phenyl acetate 165 μM in Tris HCl buffer, 0.1 M and pH 7, with 1% (v/v) Triton X-100) and 0.1 ml lipase is incubated at 40?C during 10 minutes and the absorbance was measured at 750 nm. Linearity was observed in the concentration range 0-0.8 g/L lipase.