TITLE:
Annonaa muricata Linn Leaf Induce Apoptosis in Cancer Cause Virus
AUTHORS:
Okid Parama Astirin, Anief Nur Artanti, Meutia Srikandi Fitria, Eva Agustina Perwitasari, Adi Prayitno
KEYWORDS:
Chemoprevention; Annona muricata Linn Leaf; HeLa Cell Culture; Apoptosis
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.4 No.7,
September
4,
2013
ABSTRACT: Introduction: Now many studies conducted on the drug substance from nature that can serve as
an anticancer agent as a potential chemoprevention agent, such as Annona
muricata Linn leaf escort chemotherapy, which was flaring. The cancer cell in
humans was included the loss of p53 protein function due to mutations in the
protein gene. Other causes are that the p53 proteins are not functioning due to an increase in protein misfolding event
chaperones and degradation events ubiquitous as binding by viral protein. Method: Cytotoxicity assay performed on 24
well plate micro-cultures. HeLa cells are as 2 × 104 cells in 100 mL in RPMI
media. Created control is RPMI and solvent DMSO 0.25%. Cytotoxic Test performed
by the method of calculation tryphan blue dye exclusion. Being
fasted for 24 hours in
the culture medium, then the cells are grown in micro-plate with media plus samples with a non-lethal
concentration (LC50) of partition and fractionation Annona muricata Linn leaf. Sampling is performed at 24 hours. Each of these
wells is calculated the number of living cells and made the curve of cell
number and incubation time. Result: The results showed that HeLa cells are being
LC50 partition of leaves Annona muricata Linn
in ethyl acetate
his cell death rate was higher (2000 μg/ml have 131.89%; 15.625 μg/ml have 11.37%) and in ethanol-distillate water his cell death rate was lower
(2000 μg/ml have 35.80%; 15.625 μg/ml have 3.97%). Another results showed that
HeLa cells are being LC50 fractionation of leaves Annona muricata Linn in chloroform his cell death rate was higher
(2000 μg/ml have 91.86%; 15.625 μg/ml have 2.68%) and in ethyl acetate, his cell death rate was lower (2000 μg/ml have 23.79%; 15.625