TITLE:
Identification of Phenotypic and Genotypic Variability among the Isolates of Ramularia areola of Brazilian Cotton
AUTHORS:
Larissa Girotto, Mariana S. Marangoni, Janaina N. Matos, Rafael Galbieri, Wilson P. Almeida, Yeshwant R. Mehta
KEYWORDS:
Ramularia; False Mildew; Genotypic and Phenotypic Differentiation; Gossypium hirsutum
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.9,
August
30,
2013
ABSTRACT: Genotypic and
phenotypic variation among 16 isolates of Ramularia
areola of Gossypium hirsutum collected from five different geographical regions of Brazil was studied
through virulence spectrum on three cultivars in the glasshouse and through
ERIC- and REP-PCR and ITS1-5.8S-ITS2 rDNA analysis. Difference in virulence
spectrum and molecular analysis of some isolates was observed. ERIC- and
REP-PCR showed similar results and revealed a high level of diversity among the
isolates. A unique profile for both ERIC and REP was obtained for most
isolates. On the other hand, the ITS rDNA analysis did not show different
PCR-RFLP patterns. While some isolates differed among each other considering
genotypic and phenotypic reactions, no clear cut evidence was found about the
existence of genetic lineages of R.
areola in Brazil.
Identification of genetic variability among the R. areola isolates originated from different geographic regions
would permit screening of Brazilian germplasm and achieve sources with a wide
spectrum of resistance. This is the first report of the genotypic and
phenotypic variability among the R.
areola isolates originated from five cotton growing regions of Brazil.