TITLE:
Proteomics-Based Analysis of Phalaenopsis amabilis in Response toward Cymbidium Mosaic Virus and/or Odontoglossum Ringspot Virus Infection
AUTHORS:
Tongfei Lai, Yanfu Deng, Pengchen Zhang, Zhijuan Chen, Feng Hu, Qi Zhang, Yihua Hu, Nongnong Shi
KEYWORDS:
Phalaenopsis amabilis; Two-Dimensional Electrophoresis; Mass Spectrometry; Cymbidium Mosaic Virus; Odontoglossum Ringspot Virus
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.9,
August
30,
2013
ABSTRACT: Stress response
at the protein level to viral infection in orchid plants has not been extensively
investigated to date. To understand the proteomic basis of Phalaenopsis amabilis’s responses to Cymbidium Mosaic virus (CymMV), and/or Odontoglossum ring spot virus (ORSV), the total proteins were extracted from Phalaenopsis amabilis leaves infected with CymMV, ORSV, or both
respectively. Differentially expressed proteins were identified by
two-dimensional electrophoresis, and 27 of these proteins that had significant
changes were further examined by mass spectrometry. Comparing CymMV-infected
leaves with mock-inoculated ones, 2 proteins were significantly up-regulated, 9 were significantly down-regulated
and 1 previously undetected protein was identified. 10 proteins were
significantly up-regulated, 3 significantly down-regulated and 1 previously
undetected protein was identified in ORSV-infected leaves. 6 proteins were
significantly up-regulated and 9 significantly down-regulated proteins were
found in co-infected leaves. These identified proteins are involved in disease
resistance, stress response, transcriptional regulation, energy metabolism, protein
modification and the previously unknown proteins were not involved with known protein pathways.
Proteins significantly up-regulated were ATP
sulfurylase, down-regulated proteins included glutamate decarboxylase isozyme 2, RNA polymerase alpha
subunit and chloroplastic peptide deformylase 1A were proteins with similar alteration trend after all
infection treatments. Significantly up-regulated were Thioredoxin H-type and down-regulated
Cytosolic phosphoglycerate
kinase I which were
proteins that have
been shown to be specifically regulated by the infection with CymMV.
Significantly up-regulated were proteins like Rubisco large subunit, Triosephosphate isomerase,
NADP-specific isocitrate dehydrogenase and Cinnamoyl CoA
reductase CCR2 by the infection of ORSV. Protein regulation in coinfected leaves
followed a
pattern similar to that of any of the single virus infection results. The