TITLE:
Development of a SCAR Marker for Discrimination of a Thai Jasmine Rice (Oryza sativa L. cv. KDML105) Mutant, BKOS6, and Associated with Purple Color Trait in Thai Jasmine Rice-Related Varieties
AUTHORS:
Nuananong Semsang, Rattaporn Chundet, Boonrak Phanchisri
KEYWORDS:
SCAR Marker; KDML 105 Mutant; Low-Energy Ion Beam; Anthocyanin Accumulation; Purple Pigmented Rice
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.9,
August
29,
2013
ABSTRACT: The potential
of SCAR marker for discrimination of a Thai jasmine rice (Oryza sativa L. cv. KDML
105) mutant, BKOS6, obtained from ion-beam-induced mutation, was evaluated. The
improved rice variety, BKOS6, exhibited many remarkable characteristics which
fitted the multiple cropping system characteristics of
progressive agriculture including photoperiod insensitivity, early
flowering, short in stature, and purple pigment accumulation in pericarp. The
BKOS6 rice grain extract has already been proved that it exhibited higher
antioxidant properties than the KDML 105 and other tested rice grain extracts.
In this study, the BKOS6 specific SCAR marker was developed by HAT-RAPD
analysis of rice genomic DNA. The marker was successfully used to identify
BKOS6 variety and its hybrid varieties containing purple pigment accumulation
in plant tissues. Moreover, it was found that this marker could be used to detect other
purple pigmented rice varieties that genetically related to Thai jasmine rice.
Recently, a wide variety of anthocyanin-based foods are believed to
provide significant potential health benefits, and become more attractive.
KDML 105 is also a Thai premier fragrant rice variety which is one of the main
varieties of country’s rice export. Thus this molecular marker could be useful
for commercial and breeding purposes of BKOS6 mutant and other developed varieties from
KDML 105 which contain anthocyanin accumulation.