TITLE:
Selection of Highly Susceptible Cell Lines to Foot and Mouth Disease Virus Infection
AUTHORS:
O. Zabal, N. Fondevila
KEYWORDS:
Foot-and-Mouth Disease; Tissue Culture; Virus Isolation
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.3 No.5,
August
29,
2013
ABSTRACT: Different non-established cultures were
examined to find those that showed high sensitivity to Foot and Mouth Disease
Virus (FMDV). Ovine kidney cultures showed high sensitivity to types A, O and
C, and were suitable to detect viral infection in samples of animal, as well as
cell culture origin. The level of detection in this system was up to ten times
higher than in BHK21 cells, which were commonly used
for FMDV isolation and production. Viral production levels in ovine kidney
cultures ranged from similar to twice as high as in BHK21. Ovine
kidney cultures maintained these characteristics for at least 18 passages,
allowing their use as an alternative system for FMDV diagnoses.