TITLE:
Development of New Strategy for Non-Antibiotic Therapy: Bovine Lactoferrin Has a Potent Antimicrobial and Immunomodulator Effects
AUTHORS:
Salama M. Abd El Hafez, Alaa B. Ismael, Manal B. Mahmoud, Abdel-Kader A. Elaraby
KEYWORDS:
Bovine Lactoferrin; Isolation; Antimicrobial and Immunomodulator Effects
JOURNAL NAME:
Advances in Infectious Diseases,
Vol.3 No.3,
August
28,
2013
ABSTRACT: The antibiotic therapy has many problems, such as
antibiotics resistance, hypersensitivity, direct toxicity, antibiotic-induced
immunosuppresion and super-infections. This is highlighting the need for a new
strategy for non-antibiotic therapy through the use of novel immunomodulators as
naturally released ones (Lactoferrin). The present study investigates the
potential of bovine lactoferrin (bLf), isolated from bovine milk whey, to
prevent Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae) and Pseudomonas aeruginosa (P.
aerogenosa) growth and then evaluates its immunomodulator effect. First,
bLf isolation was attempted from bovine milk whey using a cation exchange
chromatography by SP-Sepharose. Second, the antimicrobial activity assays were
trailed to study the antimicrobial activity of bLf. Finally, the immune effect
of bLf was studied by lymphocyte transformation test. It was found that bLf was
separated around molecular weight of 80 kDa and showed significant inhibitory
effect against E. coli followed by P. aeruginosa, S. agalactiae and S. aureus.
bLf increased lymphocyte transformations mean values in a dose dependant
manner. The highest transformations mean value was determined at 50 μg/mL. In
conclusion, these results suggest that bLf is a potent natural antimicrobials
and immunomodulator agent.