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Veitia, N., Dita, M.A., Garcia, L., Herrera, L., Bermu dez, I., Acosta, M., Clavero, J., Orellana, P., Romero, C. and Garcia, L. (2001) Use of tissue culture and the mutagenesis in vitro for the improvement of the resistance to Alternaria solani (Solanum tuberosum Lin.), to var Desiree. Biotecnología Vegetal, 1, 43-47.

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• TITLE: In vitro and greenhouse evaluation for resistance to early blight of potato isolated from Alternaria alternata

  AUTHORS: Hamid Reza Mirkarimi, Ahmad Abasi-moghadam, Javad Mozafari

  KEYWORDS: Potato; Early Blight; Alternaria; Inoculation

  JOURNAL NAME: Agricultural Sciences, Vol.4 No.9, August 27, 2013

  ABSTRACT: Early blight of potato is caused by the fungus Alternaria alternata, one of the most destructive foliar diseases, especially in hot climates under irrigation. In this study, the virus free potato seedlings were obtained from the National Plant Gene Bank of Iran and were inoculated in vitro with a culture filtrate of A. alternate. The leaflets received a 1000-μl droplet of the A. alternata culture filtrate and were inoculated by spraying with a suspension of 105 conidia/ml of isolate A. alternata in the greenhouse method. The experimental design was a completely randomized design (CRD) with three replications and seven genotypes, which have been infected with the two leaves of each iteration. In vitro selection of fungal isolates of A. alternata, chlorotic and necrotic symptoms began 1 to 2 days after inoculation, but the assessment of greenhouse symptoms appeared 6-10 days after inoculation. The area under the disease progress curve values were presented by analysis of variance (ANOVA), and they were compared using Duncan’s test (a = 0.01%). In both methods, there was a significant difference between the potato genotypes (P In vitro selection and evaluation greenhouse, Casmos were resistant to at least figure and Marfona genotype had the highest resistance.