TITLE:
In vitro and greenhouse evaluation for resistance to early blight of potato isolated from Alternaria alternata
AUTHORS:
Hamid Reza Mirkarimi, Ahmad Abasi-moghadam, Javad Mozafari
KEYWORDS:
Potato; Early Blight; Alternaria; Inoculation
JOURNAL NAME:
Agricultural Sciences,
Vol.4 No.9,
August
27,
2013
ABSTRACT: Early blight of
potato is caused by the fungus Alternaria alternata, one of the most
destructive foliar diseases, especially in hot climates under irrigation. In
this study, the virus free potato seedlings were obtained from the National
Plant Gene Bank of Iran and were inoculated in
vitro with a culture filtrate of A. alternate. The leaflets received
a 1000-μl droplet of the A. alternata culture filtrate and were inoculated
by spraying with a suspension of 105 conidia/ml of isolate A.
alternata in the greenhouse method. The experimental design was a
completely randomized design (CRD) with three replications and seven genotypes,
which have been infected with the two leaves of each iteration. In vitro selection of fungal isolates of A. alternata, chlorotic and necrotic symptoms began 1 to 2 days after
inoculation, but the assessment of greenhouse symptoms appeared 6-10 days
after inoculation. The area under the disease progress curve values were presented by analysis of variance (ANOVA), and
they were compared using Duncan’s test (a = 0.01%). In both methods, there was
a significant difference between the potato genotypes (P In vitro selection and evaluation
greenhouse, Casmos were resistant to at least figure and Marfona genotype had
the highest resistance.