Article citationsMore>>

C. Ortiz, D. Zhang, Y. Xie, A. E. Ribbe and D. Ben-Amotz, “Validation of the Drop Coating Deposition Raman Method for Protein Analysis,” Analytical Biochemistry, Vol. 353, No. 2, 2006, pp. 157-166. doi:10.1016/j.ab.2006.03.025

has been cited by the following article:

• TITLE: Quantification of Solid Residues by Raman Spectroscopy

  AUTHORS: Esau Araiza-Reyna, Roberto Sato-Berrú, América Vázquez-Olmos

  KEYWORDS: Quantitative Raman; Aminoacids; Glycine; Solid residues; Drop Coating Deposition Raman; Principal Component Analysis

  JOURNAL NAME: Optics and Photonics Journal, Vol.3 No.5A, August 27, 2013

  ABSTRACT: We suggest a mathematical route which has the capability to obtain quantitative information of solid residues on a substrate of aluminum, in which the evaporation of the deposited micro-drops has the form of coffee rings. In this job, the glycine aminoacid was used as probe molecule. We suppose that the number of moles present on the study sample is proportional to the Raman signal and to the experimental parameters of the used Raman system. Then, the mass and the number of molecules can be determined with these data. We showed that the mathematical expression is simple and elegant for its implementation. On the other hand, we have applied and demonstrated that the technique of principal component analysis (PCA) can be used to obtain quantitative information of the sample. Our results show quantitative analysis of glycine residues between 10-11 to 10-15 g.