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TITLE:
Hierarchy of mesenchymal stem cells: Comparison of multipotentmesenchymal stromal cells with fibroblast colony forming units
AUTHORS:
Irina N. Shipounova, Natalia A. Petinati, Alexey E. Bigildeev, Natalia V. Sats, Nina J. Drize, Larisa A. Kuzmina, Elena N. Parovichnikova, Valery G. Savchenko
KEYWORDS:
Mesenchymal Multipotent Stromal Cells (MMSC); Fibroblast Colony Forming Units (CFU-F); Real-Time Polymerase Chain Reaction; Gene Expression Level; Aging
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.6 No.8A,
August
21,
2013
ABSTRACT: The organization of the compartment of mesenchymal stem cells is still obscure. Two types of human stromal precursor cells are known. Both of them are analyzed in in vitro system: mesenchymal multipotent stromal cells (MMSC) and fibroblast colony forming units (CFU-F). The aim of this study was to compare the main characteristics of MMSC and CFU-F derived from the bone marrow of 24 healthy donors. Growth and differentiation parameters, as well as relative expression levels of different genes were analyzed in MMSC and CFU-F. MMSC were cultivated for 5 passages. CFU-F concentration was determined for each bone marrow sample. The data obtained demonstrated the heterogeneity and hierarchical organization of both studied populations of stromal precursor cells-MMSC and CFU-F. These two types of stromal precursor cells turned to be different in most parameters studied. Altogether MMSC seemed to be more immature cells than CFU-F and took up the higher position in hierarchical tree of mesenchymal stem cells. The rate of differentiation and proliferative potential decreased with the donor’s age in both populations MMSC and CFU-F.