TITLE:
A Simple and Efficient Protocol for the Mass Propagation of Vanilla planifolia
AUTHORS:
Ab Rahman Zuraida, Kamarulzaman Hassan Fatin Liyana Izzati, Othman Ayu Nazreena, Wan Sembok Wan Zaliha, Che Mohd Zain Che Radziah, Zainal Zamri, Subramaniam Sreeramanan
KEYWORDS:
Vanilla planifolia; Mass Propagation; Growth Regulator; In Vitro; BAP; NAA; IBA
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.9,
August
21,
2013
ABSTRACT: The present study
describes a direct shoot regeneration-based micropropagation procedure for Vanilla planifolia. Two types of explant (i.e. shoot apex and stem
nodal segment) were screened for their shoot induction potential following a three-month
treatment with 6-benzylaminopurine (BAP) and α-Naphthaleneacetic acid (NAA). Results
indicated that the shoot apices were poor candidates for shoot induction whereas
the stem nodal segments showed potential for shoot initiation at a rate of up to
6 shoots/explant. Stem nodal segments were the most responsive as shoots formed
(55 shoots) directly following treatments with 1 mg/L BAP at half strength MS medium
after the third subculture. In addition, more shoots were produced on solid medium
treatments compared to the liquid medium treatments in two strengths of mediums tested.
Regenerated plantlets derived from the 1 mg/L of BAP treatment were induced to root following
a one month culture in growth regulator-free MS medium. There was 90% survival rate of the rooted plantlets
after acclimatization in the greenhouse. The findings in the present study
would be helpful for large-scale mass propagation of Vanilla planifolia using this simple and efficient protocol.