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Mendoza-Palomares, C., Ferrand, A., Facca, S., Fioretti, F., Ladam, G., Kuchler-Bopp, S., Regnier, T., Mainard, D. and Benkirane-Jessel, N. (2012) Smart hybrid materials equipped by nanoreservoirs of therapeutics. ACS Nano, 6, 483-490. tdoi:10.1021/nn203817t
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TITLE:
Nanostructured hybrid materials for bone-tooth unit regeneration
AUTHORS:
Sabine Kuchler-Bopp, Thibault Bécavin, Tunay Kökten, Florence Fioretti, Etienne Deveaux, Nadia Benkirane-Jessel, Laetitia Keller
KEYWORDS:
Bone; Nanostructured Material; Osteoblast; Tissue Engineering; Tooth
JOURNAL NAME:
Open Journal of Regenerative Medicine,
Vol.2 No.3,
August
21,
2013
ABSTRACT: As a part of regenerative medicine,
biomaterials are largely used in this field of nanotechnology and tissue
engineering research. We have recently developed a new scaffold using
electrospun nanofibers of Poly (ε-caprolactone),
PCL which is able to mimic the collagen extracellular matrix of cells. The aim
of this study was to engineer a biological and implantable structure leading
the regeneration of the tooth-bone unit. For this aim, we have cultured mouse
osteoblasts embedded in a collagen gel on the nanofibrous membrane and
coupled this structure with an embryonic dental germ before implantation. To
follow bone and tooth regeneration, we have performed RT-PCR, histology and immunofluorescence analysis. We showed here that
this leaving implantable structure represents an accurate strategy for
bone-tooth unit regeneration. We report here the first demonstration of bone-tooth
unit regeneration by using a strategy based on a synthetic nanostructured
membrane. This electrospun membrane is manufactured by using an FDA approved
polymer, PCL and functionalized with osteoblasts before incorporation of the
tooth germs at ED14 (the first lower molars) to generate bone-tooth unit in vivo after implantation in mice. Our
technology represents an excellent platform on which other sophisticated
products could be based.